Epigenetic modification of rhizobial genome is essential for efficient nodulation
Epigenetic modification of rhizobial genome is essential for efficient nodulation
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DOI：
10.1016/j.bbrc.2009.08.137
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发表时间：
2009-11-13
影响因子：
3.1
通讯作者：
Abe, Tomoko
中科院分区：
文献类型：
--
作者：
Ichida, Hiroyuki;Yoneyama, Katsuyoshi;Abe, Tomoko
CCRM是唯一的细菌DNA甲基转移酶，没有相应的限制性内切酶。我们建立了一株稳定高表达中生根瘤菌CCRM的突变体MlccrM-ox，并对该菌株进行了分子和表型鉴定。在接种后7天，根瘤明显延迟，但最终在根瘤上形成的根瘤表现出至少21天的固氮能力。这些结果表明，最初的形态发生事件受到CCRM过表达的影响，细菌基因组正确的DNA甲基化模式并不是植物-微生物共生所必需的，但对于有效的结瘤是必要的。(C)2009 Elsevier Inc.保留所有权利。
CcrM is one of the solitary bacterial DNA methyltransferases which does not have corresponding restriction enzymes. We established a stable ccrM-overexpressing mutant of Mesorhizobium loti, MlccrM-OX, and performed molecular and phenotypic characterization of this strain. In the M. loti MlccrM-OX infected plants, nodulation was apparently delayed at 7 days after inoculation (dai), however, the nodules that eventually formed on the MlccrM-OX roots showed nitrogen fixing ability by at least 21 dai. These results suggest that the initial morphogenic events were affected by ccrM-overexpression and that the correct pattern of DNA methylation of the bacterial genome is not essential for plant-microbe symbiosis, but are required for efficient nodulation. (C) 2009 Elsevier Inc. All rights reserved.