EFFECT OF INTERFERON AND INTERFERON INDUCERS ON INFECTIONS WITH A NONVIRAL INTRACELLULAR MICROORGANISM, CHLAMYDIA-TRACHOMATIS
EFFECT OF INTERFERON AND INTERFERON INDUCERS ON INFECTIONS WITH A NONVIRAL INTRACELLULAR MICROORGANISM, CHLAMYDIA-TRACHOMATIS
复制标题
DOI：
10.1128/iai.3.6.825-832.1971
复制
发表时间：
1971-01-01
影响因子：
3.1
通讯作者：
GORDON, FB
中科院分区：
文献类型：
--
作者：
KAZAR, J;GILLMORE, JD;GORDON, FB
研究了小鼠干扰素(IF)对沙眼衣原体(MRC-1/G株)在同源(L-929)细胞中增殖的影响以及IF诱导剂新城疫病毒和多聚核糖核酸-多聚核糖核酸复合体(PolyI：C)对小鼠实验性气雾化C感染的影响。对沙眼衣原体(MoPn株)进行了研究。用IF处理受感染的细胞培养物，减少了含有衣原体包涵体的细胞的数量，并降低了通过感染性滴定确定的衣原体产量。在衣原体感染前6小时或18小时暴露于IF时，衣原体的生长受到抑制，在衣原体感染后的早期间隔(0或4小时)用IF处理的细胞培养物产量也略有下降。无论是用腹腔液中的吞噬细胞还是用L-92 9细胞，均未观察到IF对衣原体侵入小鼠细胞的影响，表明IF的抑制作用发生在衣原体进入宿主细胞后。另有证据表明，IF的显著作用发生在细胞内衣原体成熟的早期阶段。反复暴露于新城疫病毒气雾剂并在首次感染新城疫病毒8h后雾化吸入MoPn攻击的小鼠，在感染后3d死亡率延迟2~3d，肺实变略有减少。新城疫病毒处理组小鼠在攻击后3、6和9天从肺池中提取衣原体的产量与对照组没有显著差异。在小鼠鼻腔注射100μg PolyI：C或静脉注射200μg PolyI：C后24小时用MoPn攻击小鼠也得到了类似的结果。相比之下，在攻击前10小时或攻击后24小时静脉注射新城疫病毒0.2ml(108.3空斑形成单位)可使小鼠的死亡率增加2~3天。在所有实验中，只有在小鼠暴露于IF诱导剂后48至72小时，才能在血清或20%肺悬液中发现可检测到的IF水平。
The effect of mouse interferon (IF) on the multiplication ofChlamydia trachomatis(strain MRC-1/G) in homologous (L-929) cell cultures and the effect of the IF inducers Newcastle disease virus (NDV) and polyriboinosinic acid-polyribocytidylic acid complex (poly I:C) on the experimental infection of mice with aerosolizedC. trachomatis(strain MoPn) were investigated. Treatment of infected cell cultures with IF reduced the number of cells containing chlamydial inclusions and depressed the yield of chlamydiae as determined by titrations for infectivity. Growth of chlamydiae was reduced when cultures were exposed to IF 6 or 18 hr before infection, and slight reduction of the yield was also detectable in cell cultures treated with IF at early intervals (0 or 4 hr) after chlamydial infection. No effect of IF on penetration of chlamydiae into mouse cells was observed, whether phagocytic cells from peritoneal washings or L-929 cells were used, indicating that the inhibitory effect of IF occurs after chlamydiae enter the host cell. Additional evidence was obtained that a significant effect of IF occurs at an early stage in maturation of the intracellular chlamydiae. In mice exposed repeatedly to NDV aerosols and challenged with aerosolized MoPn 8 hr after the first exposure to NDV, mortality was delayed by 2 to 3 days and lung consolidation was slightly reduced at 3 days after infection. Yields of chlamydiae from lung pools of NDV-treated mice, taken at 3, 6, and 9 days after challenge, were not significantly different from those of controls. Similar results were obtained when mice were challenged with MoPn 8 hr after intranasal injection with 100 μg of poly I:C or 24 hr after intravenous injection with 200 μg of poly I:C. In contrast, administration of 0.2 ml of NDV (108.3plaque-forming units) intravenously 10 hr before or 24 hr after challenge with MoPn accelerated mortality of mice by 2 to 3 days. In all experiments, detectable levels of IF in sera or 20% lung suspensions were found only up to 48 to 72 hr after exposure of mice to IF inducers.