Arabidopsis RAP2.2: An Ethylene Response Transcription Factor That Is Important for Hypoxia Survival
Arabidopsis RAP2.2: An Ethylene Response Transcription Factor That Is Important for Hypoxia Survival
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DOI：
10.1104/pp.110.155077
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发表时间：
2010-06-01
期刊：
影响因子：
7.4
通讯作者：
Dolferus, Rudy
中科院分区：
文献类型：
--
作者：
Hinz, Manuela;Wilson, Iain W.;Dolferus, Rudy
拟南芥（Arabidopsis thaliana） RAP2.2 （At3g14230）是一种APETALA2/乙烯响应因子型转录因子，与水稻（Oryza sativa）耐淹基因SUB1A属于同一亚家族。RAP2.2在根中表达水平较高，在茎中表达水平较低，这是由黑暗诱导的。对乙烯信号转导突变体的效应研究和分析表明，RAP2.2是由乙烯诱导的，在乙烯控制的信号转导途径中起作用。RAP2.2的过表达导致植物在缺氧（低氧）胁迫下的存活率提高，而含有该基因T-DNA敲除的品系的存活率比野生型低。这表明RAP2.2在植物抵抗缺氧胁迫的能力中很重要。对32个低氧和乙烯相关基因表达模式的观察表明，RAP2.2仅影响部分低氧响应，尤其是对糖代谢和发酵途径酶编码基因以及乙烯生物合成基因的诱导。我们的研究结果为低氧条件下基因表达的调控提供了新的见解。照明起着重要的调节作用，并与缺氧条件交织在一起；这两种刺激可能协同作用来调节缺氧反应。
Arabidopsis (Arabidopsis thaliana) RAP2.2 (At3g14230) is an APETALA2/ethylene response factor-type transcription factor that belongs to the same subfamily as the rice (Oryza sativa) submergence tolerance gene SUB1A. RAP2.2 is expressed at constitutively high levels in the roots and at lower levels in the shoots, where it is induced by darkness. Effector studies and analysis of ethylene signal transduction mutants indicate that RAP2.2 is induced in shoots by ethylene and functions in an ethylene-controlled signal transduction pathway. Overexpression of RAP2.2 resulted in improved plant survival under hypoxia (low-oxygen) stress, whereas lines containing T-DNA knockouts of the gene had poorer survival rates than the wild type. This indicates that RAP2.2 is important in a plant's ability to resist hypoxia stress. Observation of the expression pattern of 32 low-oxygen and ethylene-associated genes showed that RAP2.2 affects only part of the low-oxygen response, particularly the induction of genes encoding sugar metabolism and fermentation pathway enzymes, as well as ethylene biosynthesis genes. Our results provide a new insight on the regulation of gene expression under low-oxygen conditions. Lighting plays an important regulatory role and is intertwined with hypoxia conditions; both stimuli may act collaboratively to regulate the hypoxic response.