Promotional effects of vasoactive intestinal peptide on the development of rodent Hertwig’s epithelial root sheath
Promotional effects of vasoactive intestinal peptide on the development of rodent Hertwig’s epithelial root sheath
复制标题
血管活性肠肽对啮齿动物Hertwig上皮根鞘发育的促进作用
DOI：
10.1111/j.1741-4520.2012.00371.x
复制
发表时间：
2012
影响因子：
1.3
通讯作者：
H Suda
中科院分区：
文献类型：
--
作者：
J Xu;N Kawashima;N Fujiwara;H Harada;MS. Ota;H Suda
赫特威格上皮根鞘(HERS)是一种双层上皮细胞鞘，位于发育中牙齿釉质器官的颈环，处于牙根形成的最前端。然而，人们对调节HERS发育的确切机制知之甚少。神经肽血管活性肠肽(VIP)参与多种组织和细胞的发育。在这项研究中，我们研究了VIP在她的发育中的作用。VIP免疫反应神经纤维见于牙髓和牙根尖周围，VIP受体1(VPAC1)在HERS中有表达。VPAC1的表达水平与HERS的发生发展相关，在出生后14天和21天表达水平升高。在体外，VIP还能促进HERS来源细胞系HERS01a细胞的增殖，并上调HERS的典型分子标志物细胞角蛋白14和波形蛋白的mRNA表达。这些结果表明，VIP可能是其发生发展的一个重要因素。
Hertwig's epithelial root sheath (HERS), a bilayered epithelial cell sheath located at the cervical loop of the enamel organ in a developing tooth, is at the forefront of root formation. However, little is known about the exact mechanisms that regulate the development of HERS. The neuropeptide vasoactive intestinal peptide (VIP) is involved in the development of various tissues and cells. In this study, we investigated the roles of VIP in HERS development. VIP‐immunoreactive nerve fibers were found in the dental pulp and around the root apex of the tooth, while the expression of VIP receptor 1 (VPAC1) was observed in HERS. The expression level of VPAC1 correlated with the development of HERS and was elevated at postnatal days 14 and 21. Usingex vivocultures of neonatal tooth germs, VIP enhanced the elongation and proliferation of HERS.In vitro, VIP also promoted the proliferation of cells from the HERS‐derived cell line, HERS01a cells, and upregulated the mRNA expression ofcytokeratin 14andvimentin(typical molecular markers of HERS) in these cells. These results suggest that VIP may be an essential factor for HERS development.