Functional studies of the Ciona intestinalis myogenic regulatory factor reveal conserved features of chordate myogenesis.
Functional studies of the Ciona intestinalis myogenic regulatory factor reveal conserved features of chordate myogenesis.
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DOI：
10.1016/j.ydbio.2013.01.033
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发表时间：
2013-04-15
影响因子：
2.7
通讯作者：
Meedel, Thomas H.
中科院分区：
文献类型：
--
作者：
Izzi, Stephanie A.;Colantuono, Bonnie J.;Sullivan, Kelly;Khare, Parul;Meedel, Thomas H.
Ci-MRF是无脊椎脊索动物海鞘中唯一的生肌调节因子（MRF）。为了研究其特性，我们开发了一种简单的体内测定法，该方法基于Ci-MRF在玻璃海鞘胚胎脊索中的错误表达。我们使用该试验来检查MRF中保守的三种结构基序的作用：在脊椎动物中称为肌源性密码的碱性结构域的丙氨酸-苏氨酸（Ala-Thr）二肽，仅在碱性结构域的N末端发现的富含半胱氨酸/组氨酸（C/H）结构域，以及称为HSP 73 III的羧基末端两亲性α-螺旋。我们表明，Ala-Thr二肽是必要的正常Ci-MRF功能，而消除C/H结构域或HSP 7III单独没有明显的影响Ci-MRF，同时损失的两个图案显着降低其活性。我们的研究还表明，CiMRF和玻璃海鞘肌钙蛋白I的一个必要的E盒之间的直接相互作用是需要这个肌肉特异性基因的表达，并且在玻璃海鞘中存在多种类型的MRF调节基因。这些研究结果是一致的MRF指导的肌发生在脊索动物中的大量保护，并首次证明了无脊椎动物的MRF的基本结构域的Ala/Thr二肽的行为作为一个生肌代码。
Ci-MRF is the sole myogenic regulatory factor (MRF) of the ascidian Ciona intestinalis, an invertebrate chordate. In order to investigate its properties we developed a simple in vivo assay based on misexpressing Ci-MRF in the notochord of Ciona embryos. We used this assay to examine the roles of three structural motifs that are conserved among MRFs: an alanine-threonine (Ala-Thr) dipeptide of the basic domain that is known in vertebrates as the myogenic code, a cysteine/histidine-rich (C/H) domain found just N-terminal to the basic domain, and a carboxy-terminal amphipathic α-helix referred to as Helix III. We show that the Ala-Thr dipeptide is necessary for normal Ci-MRF function, and that while eliminating the C/H domain or Helix III individually has no demonstrable effect on Ci-MRF, simultaneous loss of both motifs significantly reduces its activity. Our studies also indicate that direct interaction between CiMRF and an essential E-box of Ciona Troponin I is required for the expression of this muscle-specific gene and that multiple classes of MRF-regulated genes exist in Ciona. These findings are consistent with substantial conservation of MRF-directed myogenesis in chordates and demonstrate for the first time that the Ala/Thr dipeptide of the basic domain of an invertebrate MRF behaves as a myogenic code.
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影响因子：
4.6
作者：
Hinits, Yaniv;Osborn, Daniel P. S.;Hughes, Simon M.
通讯作者：
Hughes, Simon M.
DOI：
10.1073/pnas.88.13.5675
发表时间：
1991-07-01
影响因子：
11.1
作者：
BRENNAN, TJ;CHAKRABORTY, T;OLSON, EN
通讯作者：
OLSON, EN
影响因子：
4.8
作者：
Etzioni, S;Yafe, A;Fry, M
通讯作者：
Fry, M
影响因子：
4.8
作者：
Delgado-Olguin, Paul;Brand-Arzamendi, Koroboshka;Recillas-Targa, Felix
通讯作者：
Recillas-Targa, Felix
影响因子：
10.5
作者：
Gerber, AN;Klesert, TR;Tapscott, SJ
通讯作者：
Tapscott, SJ