Polymeric "smart" coatings to prevent foreign body response to implantable biosensors
Polymeric "smart" coatings to prevent foreign body response to implantable biosensors
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DOI：
10.1016/j.jconrel.2012.12.028
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发表时间：
2013-08-10
影响因子：
10.8
通讯作者：
Burgess, Diane J.
中科院分区：
文献类型：
--
作者：
Wang, Yan;Papadimitrakopoulos, Fotios;Burgess, Diane J.
用于“实时”监测的可植入葡萄糖生物传感器的应用依赖于控制传感器组织界面处的负组织反应。一种新型的聚合物涂层组成的聚（乳酸-共-乙醇酸）（PLGA）微球分散在聚（乙烯醇）（PVA）水凝胶进行了评估，与虚拟传感器作为一个“智能”的药物洗脱生物相容性涂层植入式生物传感器，以防止异物反应，从而提高传感器的性能在体内。聚合物微球在植入部位缓慢释放组织修饰药物以控制炎症和纤维包裹，而水凝胶允许分析物快速扩散到传感元件。使用模具制造工艺，用PLGA/PVA复合材料涂覆与功能性葡萄糖传感器（0.5x0.5x5 mm）尺寸相同的模拟传感器。正常和糖尿病大鼠在本研究中使用的糖尿病状态对组织传感器的相互作用的影响进行了调查。很明显，PLGA/PVA水凝胶复合材料能够在模拟传感器周围形成均匀的涂层，并在整个研究过程中（一个月）保持完整。组织样本含有假传感器，与地塞米松免费复合材料涂层表现出急性和慢性炎症，以及在正常和糖尿病大鼠纤维包囊。然而，与正常大鼠相比，糖尿病大鼠在植入无药物虚拟传感器后表现出异物反应的强度降低和延迟发作。另一方面，含有用含地塞米松的复合材料涂覆的虚拟传感器的组织保持正常（即，与未处理的组织相似），在正常和糖尿病大鼠中在一个月的时间内没有发生炎症反应或纤维包裹。证明了利用PLGA微球/PVA水凝胶复合材料作为植入式生物传感器涂层的可行性。这种聚合物复合材料是一种创新的方法来控制组织-设备界面处的异物反应，以延长生物传感器的寿命。(C)2013年爱思唯尔B。版权所有© 2016
Application of implantable glucose biosensors for "real-time" monitoring is reliant on controlling the negative tissue reaction at the sensor tissue interphase. A novel polymer coating consisting of poly(lactic-co-glycolic) acid (PLGA) microsphere dispersed in poly(vinyl alcohol) (PVA) hydrogels was evaluated in combination with dummy sensors as a "smart" drug eluting biocompatible coating for implantable biosensors to prevent the foreign body response, and thus enhance sensor performance in vivo. The polymeric microspheres slowly release tissue-modifying drugs at the implantation sites to control the inflammation and fibrous encapsulation, while the hydrogel allows rapid analyte diffusion to the sensing elements. Dummy sensors with identical dimensions to that of the functional glucose sensors (0.5x0.5x5 mm) were coated with the PLGA/PVA composites using a mold fabrication process. Both normal and diabetic rats were used in the current study to investigate the effect of the diabetic state on tissue sensor interactions. It was evident that the PLGA/PVA hydrogel composite was able to form a uniform coating around the dummy sensor and stayed intact throughout the course of the study (one month). Tissue samples containing dummy sensors that were coated with dexamethasone free composites exhibited acute and chronic inflammation as well as fibrous encapsulation in both normal and diabetic rats. However, the diabetic rats exhibited decreased intensity and delayed onset of the foreign body response following implantation of drug free dummy sensors in comparison to those of normal rats. On the other hand, tissues containing dummy sensors that were coated with dexamethasone containing composites remained normal (i.e. similar to untreated tissues), with no inflammatory reaction or fibrous encapsulation occurring over the one-month period in both the normal and diabetic rats. The feasibility of utilizing PLGA microsphere/PVA hydrogel composites as coatings for implantable biosensors was demonstrated. This polymeric composite is an innovative approach to control the foreign body reaction at the tissue-device interface to prolong biosensor lifetime. (C) 2013 Elsevier B. V. All rights reserved.