MORPHOLOGIC AND HEMODYNAMIC COMPARISON OF TUMOR AND HEALING NORMAL TISSUE MICROVASCULATURE
MORPHOLOGIC AND HEMODYNAMIC COMPARISON OF TUMOR AND HEALING NORMAL TISSUE MICROVASCULATURE
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DOI：
10.1016/0360-3016(89)90375-1
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发表时间：
1989-07-01
影响因子：
7
通讯作者：
GROSS, JF
中科院分区：
文献类型：
--
作者：
DEWHIRST, MW;TSO, CY;GROSS, JF
本研究的目的是比较肿瘤和正常肉芽组织的微血管形态和血流动力学特征，以开发可用于预测最可能与缺氧相关的微血管特征的定量数据。Fisher 344大鼠的背侧皮瓣窗口室用于在手术植入该室后2周显现10个肉芽组织和12个肿瘤（R3230 AC腺癌）组织的微脉管系统。形态测量从照片和视频技术被用来评估红细胞的速度在个别血管。两种组织的血管体积百分比接近20%，但在其他形态学和血流动力学测量中观察到显著差异。肿瘤中的单个血管尺寸（长度和直径）平均为肉芽组织中的两倍。此外，肿瘤中的红细胞速度是肉芽组织中的两倍。除了这些平均值的巨大差异外，肿瘤微血管形态学存在显著异质性，表明与肉芽组织相比空间不均匀性。血管间距的近似值表明肿瘤和肉芽组织中的平均值分别为257和118微米。肉芽组织中的血管密度是肿瘤组织中的四倍。这些结果表明，血管间距离更可能导致肿瘤缺氧，特别是考虑到该组织的侧变异性。血流分支模式的分析表明，血管分流经常发生在直径为10至90微米的血管中。本研究的结果表明，在该肿瘤模型中，与肉芽组织对照相比，存在更大程度的条件，如低血管密度、血管分流、血管长度过长和/或低红细胞速度。这些条件可能有助于缺氧的发展。
The purpose of this study was to compare microvascular morphometric and hemodynamic characteristics of a tumor and granulating normal tissue to develop quantitative data that could be used to predict microvascular characteristics which would be most likely associated with hypoxia. The dorsal flap window chamber of the Fisher 344 rat was used to visualize the microvasculature of 10 granulating and 12 tumor (R3230 AC adenocarcinoma) tissues at 2 weeks following surgical implantation of the chamber. Morphometric measurements were made from photomontages and video techniques were used to assess red cell velocities in individual vessels. The percent vascular volume of both tissues was close to 20%, but significant differences were noted in other morphometric and hemodynamic measurements. Individual vessel dimensions (length and diameter) in tumors averaged twice as large as those in granulating tissues. Furthermore, red cell velocities were twice as high in tumors as in granulating tissues. In addition to these large differences in average values, there was significant heterogeneity in tumor microvascular morphometry, indicating spatial nonuniformity compared with the granulating tissue. Approximations of vessel spacing, indicated an average of 257 and 118 microns in tumors and granulating tissues, respectively. Vessel densities were four times greater in granulating tissues than in tumor tissues. These results indicated that intervessel distances were more likely to result in hypoxia in tumors, especially considering the side variability in that tissue. Analysis of flow branching patterns showed that vascular shunts occurred frequently in vessels ranging from 10 to 90 microns in diameter. The results of this study indicate, in this tumor model, that conditions such as low vascular density, vascular shunts, excessive vascular length and/or low red cell velocity exist to a greater extent than the granulating tissue control. These conditions are likely to be conductive to the development of hypoxia.