Improved cell adhesion and osteogenesis using a PLTGA (poly L -lactide, 1,3-trimethylene carbonate, and glycolide) terpolymer by gelatin-assisted hydroxyapatite immobilization for bone regeneration
Improved cell adhesion and osteogenesis using a PLTGA (poly L -lactide, 1,3-trimethylene carbonate, and glycolide) terpolymer by gelatin-assisted hydroxyapatite immobilization for bone regeneration
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使用 PLTGA（聚 L-丙交酯、1,3-三亚甲基碳酸酯和乙交酯）三元共聚物，通过明胶辅助羟基磷灰石固定来改善细胞粘附和成骨，促进骨再生
DOI：
10.1039/c7tb02293j
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通讯作者：
Zhong-Yong Fan
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作者：
Qian Chen;Lu Cao;Jie-Lin Wang;Hang Zhao;Hong Lin;Zhong-Yong Fan;Zhong-Yong Fan
PLTGA（聚L-丙交酯、三亚甲基碳酸酯、乙交酯）复合材料具有良好的生物相容性和可控的生物降解性，在骨科领域具有巨大的应用潜力。然而，裸露的PLTGA的不利的表面条件，如其差的亲水性和光滑的形态阻碍了其临床应用，突出了需要定制的表面修饰，以改善其细胞学行为和成骨能力。本文中，我们开发了一种简便有效的策略，存款明胶/羟基磷灰石（GEL/HAP）混合涂层到表面的PLTGA，涉及连续的化学接枝和原位反应步骤。在表面改性处理之后，所得的具有GEL/HAP涂层的PLTGA支架分别表现出显著改善的亲水性（水接触角为79.1°对48.2°）和增加的表面粗糙度（均方根粗糙度为18.4对267.9 nm，超过14倍）。此外，前成骨细胞（MC 3 T3-E1）细胞接种到裸/修饰的PLGA支架上，以评估生物学性能，包括细胞粘附、增殖、矿化和成骨分化。基于这些结果，GEL/HAP混合涂层可以赋予PLTGA骨基质增强的细胞粘附、增殖和成骨功能。总之，用GEL/HAP混合涂层对PLTGA进行后处理可能是骨再生应用中一种有前途的方法。
A PLTGA (poly L-lactide, trimethylene carbonate, glycolide) terpolymer possesses great potential for orthopedic applications due to its excellent biocompatibility and controllable biodegradability. However, the unfavorable surface conditions of bare PLTGA such as its poor hydrophilicity and smooth morphology impede its clinical applications, highlighting the need for tailored surface modifications to improve its cytological behavior and osteogenic capacity. We herein develop a facile and effective strategy to deposit a gelatin/hydroxyapatite (GEL/HAP) hybrid coating onto the surface of PLTGA that involves consecutive chemical grafting and in situ reaction steps. Following the surface modification treatment, the resultant PLTGA scaffold with the GEL/HAP coating exhibited drastically improved hydrophilicity (79.1° vs. 48.2° in water contact angle) and increased surface roughness (18.4 vs. 267.9 nm, more than 14-fold, in root-mean-square roughness), respectively. In addition, preosteoblast (MC3T3-E1) cells were seeded onto the bare/modified PLTGA scaffold to evaluate biological performance, including cell adhesion, proliferation, mineralization and osteogenic differentiation. Based on these results, the GEL/HAP hybrid coating can endow the PLTGA terpolymer substrate with enhanced cell adhesion, proliferation and osteogenic functionality. Overall, post-treatment of PLTGA with the GEL/HAP hybrid coating may be a promising methodology in bone regeneration applications.