Fibroblast adhesion on ECM-derived peptide modified poly(2-hydroxyethyl methacrylate) brushes: Ligand co-presentation and 3D-localization
Fibroblast adhesion on ECM-derived peptide modified poly(2-hydroxyethyl methacrylate) brushes: Ligand co-presentation and 3D-localization
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DOI：
10.1016/j.biomaterials.2014.12.011
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发表时间：
2015-03-01
期刊：
影响因子：
14
通讯作者：
Klok, Harm-Anton
中科院分区：
文献类型：
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作者：
Desseaux, Solenne;Klok, Harm-Anton
通过甲基丙烯酸 2-羟乙酯的表面引发聚合制备的聚合物刷是制造模型生物界面以研究细胞-基质相互作用的强大平台。在这篇手稿中，表面引发聚合的多功能性和聚（2-羟乙基甲基丙烯酸酯）（PHEMA）聚合物刷平台用于解决两个基本问题，即配体共呈现和生化线索的 3D 定位对细胞行为的影响。使用一系列以不同相对表面浓度呈现 RGD 和 PHSRN 配体的 PHEMA 刷，本研究明确证明：（i）在 RGD 功能化基质上共同呈现 PHSRN 线索可增强细胞粘附；（ii）当两种配体以相同表面浓度呈现时，这种协同效应最高。在本研究的第二部分中，研究了 3T3 成纤维细胞在一系列 PHEMA 刷上的粘附，这些刷在距离细胞基质界面 12、23 或 42 nm 处呈现 RGD 配体。虽然发现细胞粘附在距界面 23 nm 处呈现细胞粘附肽的表面，但距界面 42 nm 处含有 RGD 配体的聚合物刷不支持细胞粘附。 (C) 2014 Elsevier Ltd. 保留所有权利。
Polymer brushes prepared via surface-initiated polymerization of 2-hydroxyethyl methacrylate are powerful platforms for the fabrication of model biointerfaces to study cell-substrate interactions. In this manuscript, the versatility of surface-initiated polymerization and the poly(2-hydroxyethyl methacrylate) (PHEMA) polymer brush platform are used to address two fundamental questions, viz, the effects of ligand co-presentation and of the 3D localization of biochemical cues on cell behavior. Using a series of PHEMA brushes that present RGD and PHSRN ligands in various relative surface concentrations, the present study unequivocally demonstrates that: (i) co-presentation of PHSRN cues on an RGD functionalized substrate enhances cell adhesion and (ii) this synergetic effect is highest when the two ligands are presented at equal surface concentrations. In the second part of this study, adhesion of 3T3 fibroblasts on a series of PHEMA brushes that present the RGD ligand at a distance of 12, 23 or 42 nm away from the cell substrate interface is investigated. While cells were found to adhere to surfaces that presented the cell adhesive peptides at distances up to 23 nm from the interface, polymer brushes that contained the RGD ligands 42 nm away from the interface did not support cell adhesion. (C) 2014 Elsevier Ltd. All rights reserved.