Soft X-ray diffraction microscopy of a frozen hydrated yeast cell.
Soft X-ray diffraction microscopy of a frozen hydrated yeast cell.
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DOI：
10.1103/physrevlett.103.198101
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发表时间：
2009-11-06
影响因子：
8.6
通讯作者：
Jacobsen C
中科院分区：
文献类型：
--
作者：
Huang X;Nelson J;Kirz J;Lima E;Marchesini S;Miao H;Neiman AM;Shapiro D;Steinbrener J;Stewart A;Turner JJ;Jacobsen C
我们报告的第一个图像的完整，冷冻水合真核细胞使用x射线衍射显微镜，或相干x射线衍射成像。通过将样品在液态乙烷中冷冻并保持在- 170°C以下，由于脱水，冰结晶和辐射损伤而产生的伪影大大减少。在这个例子中，使用520 eV x射线记录和重建相干衍射数据，以优于25 nm的分辨率显示出芽酵母细胞。该演示代表了在自然水合状态下对细胞进行高分辨率成像的重要一步，而不受x射线光学的限制。
We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below −170 °C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.