Development and characterization of 45 novel microsatellite markers for sea cucumber (Apostichopus japonicus)
Development and characterization of 45 novel microsatellite markers for sea cucumber (Apostichopus japonicus)
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DOI：
10.1111/j.1471-8286.2007.01876.x
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发表时间：
2007-11-01
期刊：
影响因子：
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通讯作者：
Hu, Jingjie
中科院分区：
文献类型：
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作者：
Zhan, Aibin;Bao, Zhenmin;Hu, Jingjie
采用简单重复序列富集文库筛选和表达序列标签数据库挖掘技术开发仿刺参微卫星标记。选择83个微卫星位点对48名个体进行多态性评估。结果表明，45个新位点具有多态性。等位基因数在2 ~ 16个之间，观察杂合度在0 ~ 0.9375之间，期望杂合度在0.1135 ~ 0.9674之间。结果表明，在所有的基因座中，没有发现显著的连锁不平衡，有26个基因座符合Hardy-Weinberg平衡。因此，这些标记是研究群体结构和构建连锁图谱的潜在工具。- 是的
Simple sequence repeat-enriched library screening and expressed sequence tag database mining were adopted to develop microsatellite markers for sea cucumber (Apostichopus japonicus). Eighty-three microsatellite loci were selected for polymorphism assessment using 48 individuals. The results showed that 45 novel loci were polymorphic. The number of alleles ranged from two to 16, and the values of observed and expected heterozygosities varied from 0 to 0.9375 and from 0.1135 to 0.9674, respectively. No significant linkage disequilibrium between pairs of loci was found and 26 loci conformed to the Hardy-Weinberg equilibrium. These markers are therefore a potential tool for studies in the population structure and linkage map construction for A. japonicus.