Interaction of the bacteriophage phi 29 connector protein with the viral DNA.
Interaction of the bacteriophage phi 29 connector protein with the viral DNA.
复制标题
噬菌体 phi 29 连接器蛋白与病毒 DNA 的相互作用。
DOI：
10.1016/0042-6822(86)90191-1
复制
发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
Carrascosa,JL
中科院分区：
文献类型：
--
作者：
Herranz,L;Salas,M;Carrascosa,JL
形成噬菌体φ29 （p10）连接体的蛋白质与DNA结合。显然，p10结合不是序列特异性的。然而，在φ29 DNA末端共价连接的末端蛋白（p3）的存在使结合在DNA末端的p10分子显著增加，这表明末端蛋白介导了噬菌体连接器对DNA末端的识别。由于p3-DNA复合物是噬菌体φ29 DNA封装的底物，这些结果可能反映了噬菌体连接器在封装过程中的直接含义。
The protein that forms the connector of phage φ29, p10, binds to DNA. Apparently, p10 binding is not sequence specific. Nevertheless, the presence of the terminal protein (p3) covalently attached to the ends of φ29 DNA produces a significant increase of p10 molecules bound to the DNA ends, thus suggesting a terminal protein-mediated recognition of DNA ends by the phage connector. As the p3-DNA complex is the substrate for phage φ29 DNA packaging, these results may reflect a direct implication of the phage connector in the packaging process.