Use of photopolymerizable membranes based on polyacrylamide hydrogels for enzymatic microsensor construction
Use of photopolymerizable membranes based on polyacrylamide hydrogels for enzymatic microsensor construction
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DOI：
10.1016/s0003-2670(97)00332-2
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发表时间：
1997-09-30
影响因子：
6.2
通讯作者：
KoudelkaHep, M
中科院分区：
文献类型：
--
作者：
Jimenez, C;Bartrol, J;KoudelkaHep, M
这项工作报告了基于半导体技术和光固化膜的酶微传感器的构建。平面三微电极电池和带有 Si3N4 膜的 pH 敏感离子选择场效应晶体管 (ISFET) 已分别用作葡萄糖和尿素测量的传感器。聚丙烯酰胺已被用作通过包埋酶来固定的基质材料。这种水凝胶允许通过与半导体技术兼容的光刻技术进行膜沉积和图案化。由于整个过程可能实现自动化，它还提供可重复的薄膜。为了提高传感器的长期稳定性，测试了在膜中引入戊二醛进行酶间交联。对使用和不使用交联剂的两种膜制备方法进行比较，观察到葡萄糖和尿素传感器的长期稳定性增加了大约两倍。已经研究了使用几种膜制剂的葡萄糖和尿素传感器在不同测量条件下的响应特性。电流型葡萄糖传感器的灵敏度为 45-67 nA mM(-1)，线性范围为 4x10(-3)-1 mM，具体取决于膜厚度和酶浓度。尿素酶场效应晶体管 (ENFET) 传感器在 0.2-8 mM 尿素浓度范围内获得了每十年 50 至 58 mV 的灵敏度，具体取决于膜制备。 (C) 1997 Elsevier Science B.V.
The construction of enzymatic microsensors based on semiconductor technology and photo-curable membranes is reported in this work. A planar three-microelectrode cell and a pH-sensitive Ion Selective Field-Effect Transistor (ISFET) with Si3N4 membrane have been used as transducers for glucose and urea measurements, respectively. Polyacrylamide has been employed as matrix material for the immobilization by entrapment of the enzyme. This hydrogel permits the membrane deposition and patterning by photolithographic techniques which are compatible with semiconductor technology. It also provides reproducible and thin membranes due to the possible automatization of the whole process. In order to improve the longterm stability of the sensor, the introduction of glutaraldehyde in the membrane for inter-enzyme cross-linking has been tested. Both methods of membrane preparation, with the cross-linker and without it, have been compared observing an increase of the long-term stability of around two times for glucose and urea sensors. The response characteristics of glucose and urea sensors using several membrane preparations have been studied under different measurement conditions. A sensitivity of 45-67 nA mM(-1) in a linear range 4x10(-3)-1 mM depending on the membrane thickness and enzyme concentration has been observed for the amperometric glucose sensor. A sensitivity from 50 to 58 mV per decade in a concentration range 0.2-8 mM urea depending on the membrane preparation has been obtained for the urea enzymatic Field-Effect Transistor (ENFET) sensor. (C) 1997 Elsevier Science B.V.