Characterization of the Octopaminergic and Tyraminergic Neurons in the Central Brain of Drosophila Larvae
Characterization of the Octopaminergic and Tyraminergic Neurons in the Central Brain of Drosophila Larvae
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DOI：
10.1002/cne.23616
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发表时间：
2014-10-15
影响因子：
2.5
通讯作者：
Thum, Andreas S.
中科院分区：
文献类型：
--
作者：
Selcho, Mareike;Pauls, Dennis;Thum, Andreas S.
果蝇幼虫能够根据先前的经验评估感官信息，类似于成年苍蝇，其他昆虫物种和脊椎动物。幼虫和成年苍蝇可以被教导将气味刺激与糖奖励相关联，并且先前的工作涉及章鱼胺能和多巴胺能调节系统在强化信号传导中。在这里，我们使用遗传学来分析解剖，单细胞水平，章鱼胺能/酪胺能系统在幼虫的大脑和食管下神经节。这些神经元的亚群的基因消融使我们能够确定它们对食欲嗅觉学习的必要性。这些实验表明，约39个主要形态可区分的章鱼胺能/酪胺能神经元的一个小的子集参与在果蝇幼虫大脑中的信号奖励。除了以前的工作，幼虫的运动，这些数据功能分离的章鱼胺能/酪胺能系统分为两组约40个神经元。那些位于胸/腹神经节参与幼虫的运动，而在食管下神经节和大脑半球的其他介导的奖励信号。(C)2014 Wiley Periodicals，Inc.
Drosophila larvae are able to evaluate sensory information based on prior experience, similarly to adult flies, other insect species, and vertebrates. Larvae and adult flies can be taught to associate odor stimuli with sugar reward, and prior work has implicated both the octopaminergic and the dopaminergic modulatory systems in reinforcement signaling. Here we use genetics to analyze the anatomy, up to the single-cell level, of the octopaminergic/tyraminergic system in the larval brain and subesophageal ganglion. Genetic ablation of subsets of these neurons allowed us to determine their necessity for appetitive olfactory learning. These experiments reveal that a small subset of about 39 largely morphologically distinguishable octopaminergic/tyraminergic neurons is involved in signaling reward in the Drosophila larval brain. In addition to prior work on larval locomotion, these data functionally separate the octopaminergic/tyraminergic system into two sets of about 40 neurons. Those situated in the thoracic/abdominal ganglion are involved in larval locomotion, whereas the others in the subesophageal ganglion and brain hemispheres mediate reward signaling. (C) 2014 Wiley Periodicals, Inc.