Polarimetric assessment of healthy and radiofrequency ablated porcine myocardial tissue
Polarimetric assessment of healthy and radiofrequency ablated porcine myocardial tissue
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DOI：
10.1002/jbio.201500184
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发表时间：
2016-07-01
影响因子：
2.8
通讯作者：
Vitkin, Alex
中科院分区：
文献类型：
--
作者：
Ahmad, Iftikhar;Gribble, Adam;Vitkin, Alex
射频消融为心律失常提供了一种潜在的治疗方法，在关键部位适当滴定的能量可以破坏致心律失常的灶。由此产生的消融损伤通常由核心(凝固性坏死)组成，周围环绕着混合的活细胞和非活细胞。射频损伤的范围很难用目前的成像技术来描绘。在这里，我们通过Mueller矩阵偏振测量仪在背向散射几何中探索了来自未经处理(n=5)和射频消融的猪心脏(n=5)的10个体外样品的极化特征。在正常、射频消融和边缘区域之间，除极差Delta(T)有显著差异(p<；0.01)。与健康区域相比，核心区和边缘区的线性延迟，差值显著降低(p<；0.05)。结果显示了旋光法的一种新的应用，即心肌射频消融程度的表征，包括重要病变边缘区域的可视化。射频消融损伤的猪心肌组织的白光照片(上图)和相应的去极化图(下图)。去极化对于显示病变核心和边缘是有用的。
Radiofrequency (RF) ablation offers a potential treatment for cardiac arrhythmia, where properly titrated energy delivered at critical sites can destroy arrhythmogenic foci. The resulting ablation lesion typically consists of a core (coagulative necrosis) surrounded by a rim of mixed viable and non-viable cells. The extent of the RF lesion is difficult to delineate with current imaging techniques. Here, we explore polarization signatures of ten ex-vivo samples from untreated (n = 5) and RF ablated porcine hearts (n = 5), in backscattered geometry through Mueller matrix polarimetry. Significant differences (p < 0.01) in depolarization, Delta(T), were observed between the healthy, RF ablated and rim regions. Linear retardance, delta, was significantly lower in the core and rim regions compared to healthy regions (p < 0.05). The results demonstrate a novel application of polarimetry, namely the characterization of RF ablation extent in myocardium, including the visualization of the important lesion rim region. White light photo (top) of porcine myocardium tissue with radiofrequency ablation lesion and corresponding depolarization map (bottom). Depolarization is useful for visualizing the lesion core and rim.