Acyl-CoA N-acyltransferase influences fertility by regulating lipid metabolism and jasmonic acid biogenesis in cotton.
Acyl-CoA N-acyltransferase influences fertility by regulating lipid metabolism and jasmonic acid biogenesis in cotton.
复制标题
酰基辅酶A N-酰基转移酶通过调节棉花脂质代谢和茉莉酸生物发生影响育性
DOI：
10.1038/srep11790
复制
发表时间：
2015-07-02
影响因子：
4.6
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Fu W;Shen Y;Hao J;Wu J;Ke L;Wu C;Huang K;Luo B;Xu M;Cheng X;Zhou X;Sun J;Xing C;Sun Y
棉花（Gossypiumspp.）棉花是一种重要的经济作物，棉花具有明显的杂种优势，肥力在棉花杂种优势中起着重要的作用。然而，在花药发育和育性中发挥关键作用的基因还没有很好的了解。在这里，我们报告了一个酰基辅酶A N-酰基转移酶（EC2.3; GhACNAT），在花药发育和生育力中起着关键作用。在转基因棉花中通过病毒诱导的基因沉默抑制GhACNAT（G.多毛类CV. C312）导致花药不裂，花粉囊饱满，花丝和雄蕊减少，植株不育。GhACNAT参与脂质代谢和茉莉酸（JA）的生物合成。在GhACNAT沉默植株和C312中差异表达的基因主要涉及脂代谢的催化活性和转录调节活性。与C312相比，GhACNAT基因沉默的植株中，脂质代谢和茉莉酸合成相关基因的表达水平发生了显著变化，叶片和生殖器官中茉莉酸的含量显著降低。外源茉莉酸甲酯处理挽救了GhACNAT沉默植物的花药开裂和花粉释放，并引起自交结实。GhACNAT基因可能通过调控脂类合成和JA生物合成途径在控制棉花育性中发挥重要作用。
Cotton (Gossypiumspp.) is an important economic crop and there is obvious heterosis in cotton, fertility has played an important role in this heterosis. However, the genes that exhibit critical roles in anther development and fertility are not well understood. Here, we report an acyl-CoA N-acyltransferase (EC2.3; GhACNAT) that plays a key role in anther development and fertility. Suppression ofGhACNATby virus-induced gene silencing in transgenic cotton (G. hirsutumL. cv. C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens and plant sterility. We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis. The genes differentially expressed inGhACNAT-silenced plants and C312 were mainly involved in catalytic activity and transcription regulator activity in lipid metabolism. InGhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312. Treatments with exogenous methyl jasmonate rescued anther dehiscence and pollen release inGhACNAT-silenced plants and caused self-fertility. TheGhACNATgene may play an important role in controlling cotton fertility by regulating the pathways of lipid synthesis and JA biogenesis.
登录
查看更多内容
一种同时定量多种植物激素和代谢物的便捷方法：在水稻-细菌相互作用研究中的应用
影响因子：
5.1
作者：
Liu H;Li X;Xiao J;Wang S
通讯作者：
Wang S
影响因子：
7.4
作者：
Heyndrickx, Ken S.;Vandepoele, Klaas
通讯作者：
Vandepoele, Klaas
影响因子：
4.8
作者：
Bates, Philip D.;Ohlrogge, John B.;Pollard, Mike
通讯作者：
Pollard, Mike
影响因子：
7.2
作者：
Burch-Smith, TM;Anderson, JC;Dinesh-Kumar, SP
通讯作者：
Dinesh-Kumar, SP
影响因子：
13.8
作者：
Gu, Zhouhang;Huang, Changjun;Zhou, Xueping
通讯作者：
Zhou, Xueping