Detection of anti-influenza A nucleoprotein antibodies in pigs using a commercial influenza epitope-blocking enzyme-linked immunosorbent assay developed for avian species
Detection of anti-influenza A nucleoprotein antibodies in pigs using a commercial influenza epitope-blocking enzyme-linked immunosorbent assay developed for avian species
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DOI：
10.1177/104063871002200102
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发表时间：
2010-01-01
影响因子：
1.5
通讯作者：
Zimmerman, Jeffrey J.
中科院分区：
文献类型：
--
作者：
Ciacci-Zanella, Janice R.;Vincent, Amy L.;Zimmerman, Jeffrey J.
流感病毒会引起猪的急性呼吸道疾病，其潜在的公共卫生意义值得关注。流感病毒的许多亚型已从猪身上分离出来，并且该病毒继续在猪群中进化。目前的抗体检测对抗原的识别有限，需要准确、广谱、高通量的筛选测试来检测猪群中的感染并帮助实施或控制措施。在当前的研究中，评估了一种商业阻断酶联免疫吸附测定（ELISA），用于检测禽类中的甲型流感病毒核蛋白抗体，以检测猪中的抗流感血清抗体。用于评估测试的血清样本是美国农业部农业研究服务处国家动物疾病中心进行的流感研究的存档样本，包括来自接种流感的猪（H1N1、H1N2、H2N3和H3N2）、接触感染猪、接种疫苗的猪和阴性对照的样本。基于已知状态的样本 (n = 453)，ELISA 结果的受试者工作特征 (ROC) 曲线分析估计优化的诊断灵敏度和特异性分别为 96.6%（95% 置信区间 [CI]：92.3、98.9）和 99.3%（95% CI：97.6、99.9）。通过使用 ROC 分析中确定的截止值，对感染 2009 年大流行 H1N1 病毒的 2 种分离株的猪进行了评估。总体而言，该测定对所研究的一系列流感亚型表现出出色的诊断性能，并且可以作为猪的有用筛查测定。
Influenza virus causes acute respiratory disease in pigs and is of concern for its potential public health significance. Many subtypes of Influenza virus have been isolated from pigs, and the virus continues to evolve in swine populations. Current antibody assays have limited antigenic recognition, and accurate, broad-spectrum, high through-put screening tests are needed to detect infections in swine herds and to aid in the implementation or control measures. In the current study, a commercial blocking enzyme-linked immunosorbent assay (ELISA) developed for the detection of Influenza A virus nucleoprotein antibodies in avian species was evaluated for the detection of anti-influenza serum antibodies in swine. Serum samples used to evaluate the test were archived samples from influenza research conducted at the U.S. Department of Agriculture-Agricultural Research Service-National Animal Disease Center and included samples from influenza-inoculated pigs (H1N1, H1N2, H2N3, and H3N2), contact-infected pigs, vaccinated pigs, and negative controls. Based on samples of known status (n = 453), a receiver operating characteristic (ROC) curve analysis of the ELISA results estimated the optimized diagnostic sensitivity and specificity at 96.6% (95% confidence interval [CI]: 92.3, 98.9) and 99.3% (95% CI: 97.6, 99.9), respectively. By using the cutoff established in the ROC analysis, the assay was evaluated in pigs infected with 2 isolates of the 2009 pandemic H1N1 virus. Overall, the assay showed excellent diagnostic performance against the range of influenza subtypes investigated and could serve as a useful screening assay for swine.