EXTENSIVE DYE COUPLING BETWEEN RAT NEOCORTICAL NEURONS DURING THE PERIOD OF CIRCUIT FORMATION
EXTENSIVE DYE COUPLING BETWEEN RAT NEOCORTICAL NEURONS DURING THE PERIOD OF CIRCUIT FORMATION
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DOI：
10.1016/0896-6273(93)90246-n
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发表时间：
1993-01-01
期刊：
影响因子：
16.2
通讯作者：
KATZ, LC
中科院分区：
文献类型：
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作者：
PEINADO, A;YUSTE, R;KATZ, LC
将一种低分子量细胞内示踪剂 Neurobiotin 注射到出生后 5-18 天制作的活体大鼠新皮质切片中的单个神经元中。第 5 天到第 12 天之间，66% 的单神经元注射标记了注射细胞周围多达 80 个神经元的簇。神经元之间的耦合主要通过树突发生。在氟烷（一种间隙连接阻断剂）存在下进行的注射消除了示踪剂向周围神经元的扩散，这意味着间隙连接介导了耦合。第 16 天后进行的注射导致很少或没有染料偶联。我们得出的结论是，发育中皮层中通过间隙连接进行的瞬时局部耦合可能提供一种用于传达细胞间信号（包括阈下电活动）的途径，从而在电路形成过程中实现局部神经元群的时间协调。
A low molecular weight intracellular tracer, Neurobiotin, was injected into single neurons in living slices of rat neocortex made at postnatal days 5-18. Between days 5 and 12, 66% of single-neuron injections labeled clusters of up to 80 neurons surrounding the injected cell. Coupling between neurons occurred primarily through dendrites. Injections done in the presence of halothane, a gap junction blocker, abolished the spread of tracer to surrounding neurons, implying that gap junctions mediate coupling. Injections done after day 16 resulted in little or no dye coupling. We conclude that transient local coupling via gap junctions in developing cortex may provide a pathway for communicating intercellular signals, including subthreshold electrical activity, and thereby enable temporal coordination of local neuronal ensembles during circuit formation.