Chloroplast DNA variations in Mediterranean olive
Chloroplast DNA variations in Mediterranean olive
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DOI：
10.1080/14620316.2008.11512346
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发表时间：
2008-01-01
影响因子：
1.9
通讯作者：
Besnard, Guillaume
中科院分区：
文献类型：
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作者：
Besnard, Guillaume
十多年来，几个欧洲团队研究了橄榄叶绿体基因组(CpDNA)的多态。检测到的cpDNA多态水平特别低；但在最近一项基于14棵地中海树木的研究中，据报道，一个基因间(trnD-trnT)间隔区的变异足以区分栽培橄榄中的至少六种单倍型。与以前的研究相比，所描述的多态水平(9个替换和7个短Indels在大约.1克朗)特别高。为了扩大分析的样本，从18棵橄榄树上产生了新的trnD-trnT序列，这些橄榄树来自地中海盆地、马卡洛尼亚、阿尔及利亚南部、肯尼亚、也门、留尼汪岛、夏威夷、伊朗和中国。在这个扩大的样本中只检测到两个碱基替换，其中一个可以区分出油橄榄亚种。鹰嘴豆和其他橄榄亚种。还发现了(PolyT)微卫星基序的长度变异。橄榄远缘居群之间的变异相对较小，这表明测序问题可能是先前报道的trnD-trnT间隔区高度多态的根源。此外，对55棵地中海橄榄树的PolyT基序的长度多态性进行了研究，此前已对其他cpDNA多态性进行了分析。在地中海橄榄中发现了两个等位基因，但它们不允许检测到新的cpDNA单倍型。建议正确分析地中海橄榄的cpDNA多态，应结合使用7个标记，以检测其正确的系统地理结构。
Over ten years, several European teams have characterised polymorphism in the olive chloroplast genome (cpDNA). A particularly low level of cpDNA polymorphism was detected; but, in a recent study based on 14 Mediterranean trees, an intergenic (trnD-trnT) spacer was reported to be sufficiently variable to distinguish at least six haplotypes in cultivated olive. Compared to previous studies, the level of polymorphism described (nine substitutions and seven short indels on a segment of approx. 1 kbp) was particularly high. In order to enlarge upon the sample analysed, new trnD-trnT sequences were generated from 18 olive trees originating from the Mediterranean Basin, Macaronesia, S. Algeria, Kenya, Yemen, Reunion Island, Hawaii, Iran, and China. Only two base substitutions were detected in this enlarged sample, one of which discriminated between Olea europaea subsp. cuspidata and other olive sub-species. Length variation at a (poly-T) microsatellite motif was also found. The relatively low variation revealed between distant populations of olive suggests that sequencing problems might be the origin of the high polymorphism previously reported in the trnD-trnT spacer. Furthermore, length polymorphism at the poly-T motif was investigated in 55 Mediterranean olive trees which had previously been analysed for other cpDNA polymorphisms. Two alleles were found in Mediterranean olive, but they did not permit detection of new cpDNA haplotypes. It is proposed that proper analyses of cpDNA polymorphism in Mediterranean olive should use a combination of seven markers in order to detect its correct phylogeographic structure.