Genome-wide association mapping for female fertility traits in Danish and Swedish Holstein cattle
Genome-wide association mapping for female fertility traits in Danish and Swedish Holstein cattle
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DOI：
10.1111/j.1365-2052.2010.02064.x
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发表时间：
2010-12-01
期刊：
影响因子：
2.4
通讯作者：
Lund, M. S.
中科院分区：
文献类型：
--
作者：
Sahana, G.;Guldbrandtsen, B.;Lund, M. S.
采用混合模型分析丹麦和瑞典荷斯坦牛生育性状QTL，进行全基因组关联研究。该分析纳入了2,531头后代测试公牛，共使用了29个牛常染色体上的36387个SNP标记。分析了11个育性性状的SNP关联。此外，混合模型分析用于关联分析，其中多基因效应被拟合为随机效应，并且单个SNP的基因型被连续地包括为模型中的固定效应。应用多重检验的Bonferroni校正来调整显著性阈值。74个SNP-性状组合显示出全染色体显著性，其中5个具有显著的全基因组显著性。在14条染色体上检测到24个QTL区域。在第3、5、10、13、19、20和24号染色体上观察到影响育性性状的QTL存在的强有力证据。QTL的间隔一般小于那些在早期的连锁研究中所描述的。鉴定育性性状相关的SNPs和定位相应的QTL在小的染色体区域，将有助于候选基因和候选多态性的搜索。
A genome-wide association study was conducted using a mixed model analysis for QTL for fertility traits in Danish and Swedish Holstein cattle. The analysis incorporated 2,531 progeny tested bulls, and a total of 36 387 SNP markers on 29 bovine autosomes were used. Eleven fertility traits were analyzed for SNP association. Furthermore, mixed model analysis was used for association analyses where a polygenic effect was fitted as a random effect, and genotypes at single SNPs were successively included as a fixed effect in the model. The Bonferroni correction for multiple testing was applied to adjust the significance threshold. Seventy-four SNP-trait combinations showed chromosome-wide significance, and five of these were significant genome-wide. Twenty-four QTL regions on 14 chromosomes were detected. Strong evidence for the presence of QTL that affect fertility traits were observed on chromosomes 3, 5, 10, 13, 19, 20, and 24. The QTL intervals were generally smaller than those described in earlier linkage studies. The identification of fertility trait-associated SNPs and mapping of the corresponding QTL in small chromosomal regions reported here will facilitate searches for candidate genes and candidate polymorphisms.