A comparative study of the human urinary mycotoxin excretion patterns in Bangladesh, Germany, and Haiti using a rapid and sensitive LC-MS/MS approach
A comparative study of the human urinary mycotoxin excretion patterns in Bangladesh, Germany, and Haiti using a rapid and sensitive LC-MS/MS approach
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DOI：
10.1007/s12550-015-0223-9
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发表时间：
2015-08-01
影响因子：
3
通讯作者：
Humpf, Hans-Ulrich
中科院分区：
文献类型：
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作者：
Gerding, Johannes;Ali, Nurshad;Humpf, Hans-Ulrich
一种改进的“稀释和射击”LC-MS/MS多生物标志物方法被用来监测23种真菌毒素及其代谢物在亚洲（孟加拉国），欧洲（德国）和加勒比地区（海地）人群中的尿液排泄。脱氧雪腐镰刀菌烯醇（DON），脱氧雪腐镰刀菌烯醇-3-葡糖苷酸（DON-3-GlcA）、T-2-毒素（T-2）、HT-2-毒素（HT-2）、HT-2-毒素-4-葡糖苷酸（HT-2-4-GlcA）、伏马菌素B-1（FB 1）、黄曲霉毒素（AF B（1）、AF B（2）、AFG（1）、AFG（2）、AFM（1））、玉米赤霉烯酮（ZEA）、玉米赤霉酮（ZAN）、它们的尿代谢物α-玉米赤霉醇和β-玉米赤霉醇（β-ZEL）和相应的14-O-葡萄糖醛酸缀合物（ZEA-14-GlcA，ZAN-14-GlcA，β-ZEL，α/β-ZEL-14-GlcA），赭曲霉毒素A（OTA），和赭曲霉毒素α（OT α）以及恩镰孢菌素B（EnB）和二氢桔霉素酮（DH-CIT）在这些化合物中。检测到8种尿霉菌毒素生物标志物（AFM（1）、DH-CIT、DON、DON-GLcA、EnB、FB 1、OTA和α-ZEL）。DON和DON-GlcA仅在来自德国和海地的尿液中检测到，而孟加拉国样本中的尿OTA和DH-CIT浓度显著较高。AFM（1）仅在孟加拉国和海地的样品中存在。通过计算可能的每日摄入量（PDI）来估计暴露量，估计值表明偶尔的毒素摄入量超过了规定的每日耐受摄入量（TDI）。通过基于生物标志物的方法检测个体真菌毒素暴露是对食品供应中真菌毒素含量的经典监测的有意义的补充。
An improved "dilute and shoot" LC-MS/MS multibiomarker approach was used to monitor urinary excretion of 23 mycotoxins and their metabolites in human populations from Asia (Bangladesh), Europe (Germany), and the Caribbean region (Haiti). Deoxynivalenol (DON), deoxynivalenol-3-glucuronide (DON-3-GlcA), T-2-toxin (T-2), HT-2-toxin (HT-2), HT-2-toxin-4-glucuronide (HT-2-4-GlcA), fumonisin B-1 (FB1), aflatoxins (AFB(1), AFB(2), AFG(1), AFG(2), AFM(1)), zearalenone (ZEA), zearalanone (ZAN), their urinary metabolites alpha-zearalanol alpha-ZEL) and beta-zearalanol (beta-ZEL), and corresponding 14-O-glucuronic acid conjugates (ZEA-14-GlcA, ZAN-14-GlcA, beta-ZEL, alpha/beta-ZEL-14-GlcA), ochratoxin A (OTA), and ochratoxin alpha (OT alpha) as well as enniatin B (EnB) and dihydrocitrinone (DH-CIT) were among these compounds. Eight urinary mycotoxin biomarkers were detected (AFM(1), DH-CIT, DON, DON-GLcA, EnB, FB1, OTA, and alpha-ZEL). DON and DON-GlcA were exclusively detected in urines from Germany and Haiti whereas urinary OTA and DH-CIT concentrations were significantly higher in Bangladeshi samples. AFM(1) was present in samples from Bangladesh and Haiti only. Exposure was estimated by the calculation of probable daily intakes (PDI), and estimates suggested occasional instances of toxin intakes that exceed established tolerable daily intakes (TDI). The detection of individual mycotoxin exposure by biomarker-based approaches is a meaningful addition to the classical monitoring of the mycotoxin content of the food supply.