Fgf19 regulated by Hh signaling is required for zebrafish forebrain development
Fgf19 regulated by Hh signaling is required for zebrafish forebrain development
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DOI：
10.1016/j.ydbio.2005.09.042
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发表时间：
2005-12-01
影响因子：
2.7
通讯作者：
Itoh, N
中科院分区：
文献类型：
--
作者：
Miyake, A;Nakayama, Y;Itoh, N
成纤维细胞生长因子信号在脑发育中起着重要作用，Fgf3和Fgf8在前脑和后脑的形成中起着至关重要的作用。FGF8也是形成中脑所必需的。在这里，我们鉴定了斑马鱼FGF19，并通过下调FGF19功能来研究其在脑发育中的作用。我们发现在胚胎脑发育过程中，FGF19在前脑、中脑和后脑表达，参与细胞增殖和细胞存活。FGF19对腹侧端脑和间脑的发育也是必需的。区域规格与小区类型规格相关联。FGF19对腹侧端脑和间脑产生的GABA能中间神经元和少突胶质细胞也是必需的。成纤维细胞生长因子和促肾上腺皮质激素信号之间的相互作用对大脑发育至关重要。在前脑，FGF19的表达在抑制HH时表达下调，但不下调Fgf3/Fgf8的表达，FGF19的过度表达部分挽救了HH抑制的表型。本研究结果表明，FGF19信号通过与HH的相互作用对前脑发育起着至关重要的作用，并为了解FGF19信号在脑发育中的作用提供了新的见解。(C)2005 Elsevier Inc.保留所有权利。
Fibroblast growth factor (Fgf) signaling plays important roles in brain development, Fgf3 and Fgf8 are crucial for the formation of the forebrain and hindbrain. Fgf8 is also required for the midbrain to form. Here, we identified zebrafish Fgf19 and examined its roles in brain development by knocking down Fgf19 function. We found that Fgf19 expressed in the forebrain, midbrain and hindbrain was involved in cell proliferation and cell survival during embryonic brain development. Fgf19 was also essential for development of the ventral telencephalon and diencephalon. Regional specification is linked to cell type specification. Fgf19 was also essential for the specification of gamma-aminobutyric acid (GABA)ergic interneurons and oligodendrocytes generated in the ventral telencephalon and diencephalon. The cross talk between Fgf and Hh signaling is critical for brain development. In the forebrain, Fgf19 expression was down-regulated on inhibition of Hh but not of Fgf3/Fgf8, and overexpression of Fgf19 rescued partially the phenotype on inhibition of Hh. The present findings indicate that Fgf19 signaling is crucial for forebrain development by interacting with Hh and provide new insights into the roles of Fgf signaling in brain development. (C) 2005 Elsevier Inc. All rights reserved.