Intracellular calcium oscillations regulate ciliary beat frequency of airway epithelial cells
Intracellular calcium oscillations regulate ciliary beat frequency of airway epithelial cells
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DOI：
10.1054/ceca.1999.0060
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发表时间：
1999-09-01
期刊：
影响因子：
4
通讯作者：
Sanderson, MJ
中科院分区：
文献类型：
--
作者：
Evans, JH;Sanderson, MJ
The effect of ATP-induced Ca2+ oscillations on ciliary activity was examined in airway epithelial cells by simultaneously measuring the ciliary beat frequency (CBF) and the intracellular Ca2+ concentration ([Ca2+](i)) near the base of the cilia. Exposure to extracellular ATP (ATP(o)) induces a rapid and large increase in both [Ca2+](i) and CBF, followed by oscillations in [Ca2+](i) and a sustained elevation in CBF. After each Ca2+ oscillation, the [Ca2+](i) returned to near basal values. By contrast, the CBF remained elevated during these Ca2+ oscillations, although each Ca2+ oscillation induced small variations in CBF. During Ca2+ oscillations, increases in CBF closely followed the rising phase of increases in [Ca2+](i), but declines in CBF lagged behind declines in [Ca2+](i). Higher frequency Ca2+ oscillations reduced variations in CBF, producing a stable and sustained elevation in CBF. The maximal CBF was induced by Ca2+ oscillations and was 15% greater than the CBF induced by the substantially larger initial [Ca2+](i) increase. These data demonstrate that the rate of CBF is not directly dependent on the absolute [Ca2+](i), but is dependent on the differential changes in [Ca2+](i) and suggest that CBF in airway epithelial cells is regulated by frequency-modulated Ca2+ signaling. (C) Harcourt Publishers Ltd 1999.
