EMMPRIN and its ligand Cyclophilin A as novel diagnostic markers in inflammatory cardiomyopathy
EMMPRIN and its ligand Cyclophilin A as novel diagnostic markers in inflammatory cardiomyopathy
复制标题
DOI：
10.1016/j.ijcard.2011.06.049
复制
发表时间：
2013-03-10
影响因子：
3.5
通讯作者：
May, Andreas E.
中科院分区：
文献类型：
--
作者：
Seizer, Peter;Geisler, Tobias;May, Andreas E.
背景：在炎症性心肌病中，基质金属蛋白酶在心脏重塑中起着关键作用。本研究的目的是调查是否“细胞外基质金属蛋白酶诱导剂”EMMPRIN（CD 147）及其配体亲环素A（CyPA）上调炎症性心肌病，并可能作为诊断标志物。因此，一系列的102人endomyoprotein活检进行了分析EMMPRIN和CyPA的表达和相关的组织学和免疫组织学findings.Methods和结果：endomyoprotein活检进行了染色EMMPRIN和CyPA除了标准的组织学（HE，Trichrom）和免疫组织学染色（MHC-II，CD 68，CD 3）。39例（38.2%）活检符合炎性心肌病的免疫组织学标准。EMMPRIN主要在心肌细胞上表达，与正常组织病理学结果相比，在非炎性心肌病中轻微（但显著）上调，与非炎性心肌病和正常组织病理学相比，在炎性心肌病中高度上调。相比之下，CyPA在非炎性心肌病中没有增强的表达，而在炎性心肌病中表达高度增强，其中它与白细胞浸润密切相关。我们发现EMMPRIN和CyPA与MHC-II分子的表达有很强的相关性（相关系数分别为0.475和0.527，p
Background: During inflammatory cardiomyopathy matrix metalloproteinases are crucially involved in cardiac remodeling. The aim of the present study was to investigate whether the "extracellular matrix metalloproteinase inducer" EMMPRIN (CD147) and its ligand Cyclophilin A (CyPA) are upregulated in inflammatory cardiomyopathy and may serve as diagnostic markers. Therefore, a series of 102 human endomyocardial biopsies were analyzed for the expression of EMMPRIN and CyPA and correlated with histological and immunohistological findings.Methods and results: Endomyocardial biopsies were stained for EMMPRIN and CyPA in addition to standard histology (HE, Trichrom) and immunohistological stainings (MHC-II, CD68, CD3). 39 (38.2%) biopsies met the immunohistological criteria of an inflammatory cardiomyopathy. EMMPRIN, which was predominantly expressed on cardiomyocytes, was slightly (but significantly) upregulated in non inflammatory cardiomyopathies compared to normal histopathological findings and highly upregulated in inflammatory cardiomyopathy compared to both non inflammatory cardiomyopathy and normal histopathology. In contrast, CyPA reveals no enhanced expression in non inflammatory cardiomyopathies and a highly enhanced expression in inflammatory cardiomyopathy, where it is closely associated with leucocytes infiltrates. We found a strong correlation between both EMMPRIN and CyPA with the expression of MHC-II molecules (correlation coefficient 0.475 and 0.527, p