In-vivo PET imaging of implanted human retinal pigment epithelium cells in a Parkinson's disease rat model
In-vivo PET imaging of implanted human retinal pigment epithelium cells in a Parkinson's disease rat model
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DOI：
10.1097/mnm.0b013e3282f5d291
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发表时间：
2008-05-01
影响因子：
1.5
通讯作者：
Tian, Jiahe
中科院分区：
文献类型：
--
作者：
Wang, Ruimin;Zhang, Jinming;Tian, Jiahe
背景和目的 研究人员发现，监测体内植入细胞的分化很困难。本研究旨在表明利用正电子发射断层扫描（PET）技术可以追踪移植人视网膜色素上皮细胞（RPE细胞）对帕金森病大鼠模型的疗效。方法治疗组和对照组分别将RPE细胞或生理盐水注射到大鼠模型损伤侧纹状体中。两组患者在移植前和移植后每隔一段时间进行PET成像，使用C-11-雷氯必利和C-11-β-CFT作为标志物。并用大鼠行为观察和免疫荧光共聚焦显微镜来验证PET结果。结果PET研究显示两组纹状体损伤侧C-11-雷氯必利积累增加，C-11-β-CFT减少。治疗组移植后，C-11-雷氯必利下降，同时 C-11-β-CFT 增加。 PET 研究显示的变化与治疗动物的行为状态和共聚焦显微镜观察结果一致。结论这些结果表明，即使是临床 PET 扫描仪也可以在一定程度上提供有关帕金森病大鼠模型中 RPE 细胞的存在和体内分化的一些信息。
Background and aim Researchers find that monitoring the differentiation of implanted cells in vivo is difficult. This study was designed to show that it is possible to track the efficacy of transplanted human retinal pigment epithelial cells (RPE cells) in a rat model of Parkinson's disease by using positron emission tomography (PET).Methods RPE cells or normal saline were injected into striatum of the injured side of the rat model in treated and control groups, respectively. PET imaging of both groups was undertaken before transplantation and at intervals afterwards, using C-11-raclopride and C-11-beta-CFT as the markers. Observation of the rats' behaviour and immunofluorescence confocal microscopy were also used to prove the PET results.Results PET studies showed increased accumulation of C-11-raclopride and decreased C-11-beta-CFT in the injured side of striatum in both groups. C-11-raclopride decreased along with a concomitant increase of C-11-beta-CFT after transplantation in the treated group. The changes shown by the PET studies paralleled the behavioural states and confocal microscopy observations in the treated animals.Conclusion These results suggest that even a clinical PET scanner could, to a certain extent, provide some information on the existence and in-vivo differentiation of RPE cells in a rat model of Parkinson's disease.