Life After Death: Are Autophagy Genes Involved in Cell Death and Survival During Plant Innate Immune Responses?
Life After Death: Are Autophagy Genes Involved in Cell Death and Survival During Plant Innate Immune Responses?
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DOI：
10.4161/auto.1.3.2080
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发表时间：
2005-08
期刊：
影响因子：
13.3
通讯作者：
Montrell Seay;S. P. Dinesh-Kumar
中科院分区：
文献类型：
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作者：
Montrell Seay;S. P. Dinesh-Kumar
植物抗病性研究界一直注意到，液泡正在迅速成为细胞死亡的核心参与者。一方面，在病原体感染的细胞中，需要通过液泡加工酶（VPE）从内到外靶向破坏液泡以诱导PCD。另一方面，完整的空泡对于功能性自噬反应至关重要，以确保未感染细胞的存活。从表面上看，这两种反应似乎代表了不同的抗性机制，它们在不同的分支点上起作用，它们对液泡的使用只是巧合。然而，更仔细的研究使我们提出了一个有趣的假设，占VPE介导的PCD和自噬依赖的细胞存活的这两个相反的作用的空泡。在初始感染期间，我们提出了一种类似于酵母中的CPY运输途径的机制，其中需要选择ATG基因用于VPE运输、液泡加工和PCD的启动。在感染后期，自噬体形成、VPE前蛋白的隔离和VPE降解需要自噬特异性基因。
It has not escaped the attention of the plant disease resistance community that the vacuole is rapidly emerging as a central player in the execution of cell death. On the one hand the targeted destruction of the vacuole—from the inside out—by vacuolar processing enzymes (VPE) is required to induce PCD in pathogen-infected cells. On the other hand, an intact vacuole is vital for a functional autophagic response to ensure survival of uninfected cells. At face value, the two responses seem to represent distinct resistance mechanisms that operate at divergent branch points and their use of the vacuole merely coincidental. However, closer examination has led us to propose an interesting hypothesis that accounts for these two opposing roles of the vacuole in both VPE-mediated PCD and autophagy-dependent cell survival. During initial infection, we propose a mechanism similar to the CPY transport pathway in yeast wherein select ATG genes are needed for VPE transport, vacuolar processing and initiation of PCD. Later during infection, autophagy-specific genes are needed for autophagosome formation, sequestration of VPE preproteins and VPE degradation.