ATHB17 enhances stress tolerance by coordinating photosynthesis associated nuclear gene and ATSIG5 expression in response to abiotic stress.
ATHB17 enhances stress tolerance by coordinating photosynthesis associated nuclear gene and ATSIG5 expression in response to abiotic stress.
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DOI：
10.1038/srep45492
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发表时间：
2017-03-30
影响因子：
4.6
通讯作者：
Xiang CB
中科院分区：
文献类型：
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作者：
Zhao P;Cui R;Xu P;Wu J;Mao JL;Chen Y;Zhou CZ;Yu LH;Xiang CB
光合作用对环境胁迫敏感，必须有效地调节以响应非生物胁迫。然而，其基本机制尚未得到很好的理解。在这里，我们报告，拟南芥HOMEOBOX 17（ATHB 17），一个拟南芥HD-Zip转录因子，调节的光合作用相关的核基因（PhANG）参与光反应和ATSIG 5在响应非生物胁迫的表达。ATHB 17对阿坝和多重胁迫处理有反应。ATHB 17过表达的植物表现出增强的胁迫耐受性，而其敲除突变体比野生型更敏感。通过RNA-seq和定量实时逆转录PCR（qRT-PCR）分析，我们发现ATHB 17并不影响许多已知的胁迫响应标记基因的表达。有趣的是，我们发现ATHB 17下调了许多PhANG，并可以通过结合其启动子直接调节几种PhANG的表达。此外，我们确定了ATSIG 5，编码质体σ因子，作为ATHB 17的靶基因之一。ATSIG 5的缺失降低了耐盐性，而ATSIG 5的过表达增强了耐盐性，类似于ATHB 17。ATHB 17可以通过调节ATSIG 5正向调节许多质体编码基因（PEG）的表达。综上所述，我们的研究结果表明，ATHB 17可能通过调节PhANG和PEG的表达来响应非生物胁迫，在保护植物中发挥重要作用。
Photosynthesis is sensitive to environmental stress and must be efficiently modulated in response to abiotic stress. However, the underlying mechanisms are not well understood. Here we report that ARABIDOPSIS THALIANA HOMEOBOX 17 (ATHB17), an Arabidopsis HD-Zip transcription factor, regulated the expression of a number of photosynthesis associated nuclear genes (PhANGs) involved in the light reaction and ATSIG5 in response to abiotic stress. ATHB17 was responsive to ABA and multiple stress treatments. ATHB17-overexpressing plants displayed enhanced stress tolerance, whereas its knockout mutant was more sensitive compared to the wild type. Through RNA-seq and quantitative real-time reverse transcription PCR (qRT-PCR) analysis, we found that ATHB17 did not affect the expression of many known stress-responsive marker genes. Interestingly, we found that ATHB17 down-regulated many PhANGs and could directly modulate the expression of several PhANGs by binding to their promoters. Moreover, we identified ATSIG5, encoding a plastid sigma factor, as one of the target genes of ATHB17. Loss of ATSIG5 reduced salt tolerance while overexpression of ATSIG5 enhanced salt tolerance, similar to that of ATHB17. ATHB17 can positively modulate the expression of many plastid encoded genes (PEGs) through regulation of ATSIG5. Taken together, our results suggest that ATHB17 may play an important role in protecting plants by adjusting expression of PhANGs and PEGs in response to abiotic stresses.