Microcalorimetric study of growth of Lactococcus lactis IL1403 at low glucose concentration in liquids and solid agar gels
Microcalorimetric study of growth of Lactococcus lactis IL1403 at low glucose concentration in liquids and solid agar gels
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DOI：
10.1016/j.tca.2013.02.013
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发表时间：
2013-05-10
影响因子：
3.5
通讯作者：
Vilu, R.
中科院分区：
文献类型：
--
作者：
Kabanova, N.;Stulova, I.;Vilu, R.
利用热活性监测仪TAM III研究了乳酸乳球菌IL1403在不同低葡萄糖浓度的液体培养物和固体琼脂凝胶中的生长情况，从2 g L-1到10 g L-1，逐步增加1 g L-1。在量热测量的同时，还测量了培养基中葡萄糖和乳酸浓度的变化以及pH值，以便获得解释量热功率-时间曲线的额外信息。所得数据与计算的产热系数Y-Q可以详细分析细菌在固体琼脂凝胶中的菌落生长情况。葡萄糖浓度在低水平下的变化允许分析和描述在液体培养和固态发酵中的生长减速阶段和生长停止机制。(C) 2013 Elsevier B.V.版权所有
Growth of Lactococcus lactis IL1403 in liquid cultures and solid agar gels at different low glucose concentrations starting from 2 g L-1 up to 10 g L-1 with a stepwise increase by 1 g L-1 was studied using thermal activity monitor TAM III. In parallel to calorimetric measurements the changes of glucose and lactic acid concentrations and pH of culture media were measured in order to obtain additional information for the interpretation of calorimetric power-time curves. The data obtained together with calculated heat yield coefficient Y-Q allowed to analyze the growth of bacteria in colonies in solid agar gels in detail. The variation of glucose concentration at low levels allows analyzing and describing growth deceleration phase and growth stopping mechanisms both in liquid cultures and in solid-state fermentations. (C) 2013 Elsevier B.V. All rights reserved.