Trpc2-deficient lactating mice exhibit altered brain and behavioral responses to bedding stimuli
Trpc2-deficient lactating mice exhibit altered brain and behavioral responses to bedding stimuli
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DOI：
10.1016/j.bbr.2010.11.002
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发表时间：
2011-03-01
影响因子：
2.7
通讯作者：
Gammie, Stephen C.
中科院分区：
文献类型：
--
作者：
Hasen, Nina S.;Gammie, Stephen C.
ttpc2基因编码一种离子通道，参与信息素检测，存在于犁鼻器中。在tprc2（-/-）敲除（KO）小鼠中，母体攻击性（后代保护）受损，并且响应于雄性的雌性脑Fos表达减少。在这里，我们研究哺乳期野生型（WT）和KO小鼠的行为和大脑对不同的嗅觉/信息素线索的反应。与以前的研究一致，KO母鼠表现出降低的母性攻击和筑巢，但我们也发现了夜间护理的不足和幼鼠体重的增加。当暴露于垫料试验时，WT母鼠通常忽略干净的垫料，但掩埋来自不熟悉雄性的雄性弄脏的垫料。相比之下，KO大坝掩埋了干净和污染的垫料。WT和KO小鼠在无垫料、干净垫料或弄脏垫料的反应中发现脑Fos表达的差异。在副嗅球，一个网站的信息素信号处理，KO小鼠表现出抑制Fos激活前二尖瓣层相对于WT小鼠在响应清洁和污染的床上用品。然而，在内侧和基底外侧杏仁核，KO小鼠表现出强大的Fos反应的床上用品，这表明杏仁核的区域规范相关的信息素传感可以在KO小鼠的大脑中活跃，尽管妥协的信号从犁鼻器。总之，这些结果提供了进一步的见解复杂的方式，信息素信号调节大脑和行为的母亲。(C)2010 Elsevier B.V.保留所有权利。
The ttpc2 gene encodes an ion channel involved in pheromonal detection and is found in the vomeronasal organ. In tprc2(-/-) knockout (KO) mice, maternal aggression (offspring protection) is impaired and brain Fos expression in females in response to a male are reduced. Here we examine in lactating wild-type (WT) and KO mice behavioral and brain responses to different olfactory/pheromonal cues. Consistent with previous studies, KO dams exhibited decreased maternal aggression and nest building, but we also identified deficits in nighttime nursing and increases in pup weight. When exposed to the bedding tests, WT dams typically ignored clean bedding, but buried male-soiled bedding from unfamiliar males. In contrast, KO dams buried both clean and soiled bedding. Differences in brain Fos expression were found between WT and KO mice in response to either no bedding, clean bedding, or soiled bedding. In the accessory olfactory bulb, a site of pheromonal signal processing, KO mice showed suppressed Fos activation in the anterior mitral layer relative to WT mice in response to clean and soiled bedding. However, in the medial and basolateral amygdala, KO mice showed a robust Fos response to bedding, suggesting that regions of the amygdala canonically associated with pheromonal sensing can be active in the brains of KO mice, despite compromised signaling from the vomeronasal organ. Together, these results provide further insights into the complex ways by which pheromonal signaling regulates the brain and behavior of the maternal female. (C) 2010 Elsevier B.V. All rights reserved.