Mouse closed head injury model induced by a weight-drop device
Mouse closed head injury model induced by a weight-drop device
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DOI：
10.1038/nprot.2009.148
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发表时间：
2009-01-01
期刊：
影响因子：
14.8
通讯作者：
Shohami, Esther
中科院分区：
文献类型：
--
作者：
Flierl, Michael A.;Stahel, Philip F.;Shohami, Esther
创伤性脑损伤是年轻人死亡的主要原因。为了模拟人类闭合性脑损伤（CHI），已经开发了各种动物模型。目前广泛应用的脑损伤模型是通过侧向液体冲击、可控皮质冲击或冲击加速度来诱导脑损伤。该模型通过标准化的失重装置诱导完整颅骨的局灶性钝性损伤，而不需要损伤前的操作。由此产生的冲击在鞘内腔室引发深度神经炎症反应，具有高一致性和可重复性，导致神经损伤和血脑屏障的破坏。在本方案中，我们定义了诱导小鼠CHI的标准化程序，并通过调节体重下降高度来确定CHI的各种严重程度。在经验丰富的人员中，这种CHI模型可以在每只动物30秒内完成，随后的创伤后分析和数据收集需要额外的时间。
Traumatic brain injury represents the leading cause of death in young individuals. Various animal models have been developed to mimic human closed head injury (CHI). Widely used models induce head injury by lateral fluid percussion, a controlled cortical impact or impact acceleration. The presented model induces a CHI by a standardized weight-drop device inducing a focal blunt injury over an intact skull without pre-injury manipulations. The resulting impact triggers a profound neuroinflammatory response within the intrathecal compartment with high consistency and reproducibility, leading to neurological impairment and breakdown of the blood-brain barrier. In this protocol, we define standardized procedures for inducing CHI in mice and determine various severity grades of CHI through modulation of the weight falling height. In experienced hands, this CHI model can be carried out in as little as 30 s per animal, with additional time required for subsequent posttraumatic analysis and data collection.