Inducible gene expression systems for higher eukaryotic cells
Inducible gene expression systems for higher eukaryotic cells
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DOI：
10.1016/0958-1669(94)90067-1
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发表时间：
1994-01-01
影响因子：
7.7
通讯作者：
Bujard, Hermann
中科院分区：
文献类型：
--
作者：
Gossen, Manfred;Bonin, Angelika L.;Bujard, Hermann
在已开发的用于控制高等真核生物基因表达的各种调控系统中，那些利用原核系统的元件的调控系统目前获得了最高的特异性。特别是，基于大肠杆菌四环素耐药操纵子的抑制子/操纵子元件的系统显示出广泛的适用性。在过去的一年里，这些系统的用处不仅在细胞层面上得到了证明，而且在生物层面上也得到了证明(即在转基因动物和植物中)。
Of the wide variety of regulatory systems that have been developed to control gene expression in higher eukaryotes, those utilizing elements from prokaryotic systems presently achieve the highest specificity. In particular, systems based on the repressor/operator elements of anEscherichia colitetracycline resistance operon appear broadly applicable. During the past year, the usefulness of these systems has been demonstrated not only at the cellular level, but also at the organismal level (i.e. in transgenic animals and plants).