Immunohistochemical differential diagnosis between large cell neuroendocrine carcinoma and small cell carcinoma by tissue microarray analysis with a large antibody panel
Immunohistochemical differential diagnosis between large cell neuroendocrine carcinoma and small cell carcinoma by tissue microarray analysis with a large antibody panel
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DOI：
10.1309/dt6bj698ldx2nggx
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发表时间：
2006-05-01
影响因子：
3.5
通讯作者：
Ochiai, A
中科院分区：
文献类型：
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作者：
Nitadori, JI;Ishii, G;Ochiai, A
为了阐明大细胞神经内分泌癌 (LCNEC) 和小细胞肺癌 (SCLC) 之间的其他表型差异，我们对手术切除的 LCNEC 和 SCLC 标本进行了组织微阵列 (TMA) 分析。根据染色强度和阳性染色细胞的百分比对 48 种抗体的免疫染色进行评分。与 SCLC 相比，有四种蛋白质在 LCNEC 中显着表达：细胞角蛋白 (CK)7，113 vs 49 (P < .0301)； CK18，171 与 60 (P < .0008)； E-钙粘蛋白，77 vs 9 (P < .0073)；和 β-连环蛋白，191 vs 120 (P < .0286)。含有 LCNEC 和 SCLC 成分的横截面的免疫染色显示，与 SCLC 成分相比，3 例中有 2 例 LCNEC 成分中 CK7、CK18 和 β-catenin 显着表达。我们的结果表明，CK7、CK18、E-钙粘蛋白和 β-连环蛋白的显着表达是 LCNEC 比 SCLC 更具特征的特征，这些发现进一步支持这些肿瘤类型在形态学和免疫表型上（如果不是生物学上）是独立的实体。
To elucidate additional phenotypic differences between large cell neuroendocrine carcinoma (LCNEC) and small cell lung carcinoma (SCLC), we performed tissue microarray (TMA) analysis of surgically resected LCNEC and SCLC specimens. Immunostaining with 48 antibodies was scored based on staining intensity and the percentage of cells that stained positively. Four proteins were identified as significantly expressed in LCNEC as compared with SCLC: cytokeratin (CK)7, 113 vs 49 (P < .0301); CK18, 171 vs 60 (P < .0008); E-cadherin, 77 vs 9 (P < .0073); and beta-catenin, 191 vs 120 (P < .0286). Immunostaining of cross-sections containing LCNEC and SCLC components revealed significant expression of CK7, CK18, and beta-catenin in the LCNEC component compared with the SCLC component in 2 of 3 cases. Our results indicate that significant expression of CK7, CK18, E-cadherin, and beta-catenin is more characteristic of LCNEC than of SCLC and these findings provide further support that these tumor types are separate entities morphologically and immunophenotypically, if not biologically.