Evidence for replication of hepatitis delta virus RNA in hepatocyte nuclei after in vivo infection.
Evidence for replication of hepatitis delta virus RNA in hepatocyte nuclei after in vivo infection.
复制标题
体内感染后肝细胞核中丁型肝炎病毒RNA复制的证据。
DOI：
10.1016/0042-6822(88)90078-5
复制
发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
J. Gerin
中科院分区：
文献类型：
--
作者：
E. Gowans;B. Baroudy;F. Negro;A. Ponzetto;R. Purcell;J. Gerin
通过原位杂交检测自然感染的人类肝脏和实验感染的黑猩猩和土旱鼠肝脏，发现丁型肝炎病毒（HDV） RNA仅限于肝细胞。基因组RNA的丰度是抗基因组RNA的20-30倍，以单链RNA为主，而抗基因组RNA以双链RNA为主。在急性丁型肝炎中，病毒RNA是单细胞中比丁型肝炎抗原（HDAg）更可靠的病毒感染标志物，而在慢性肝炎中，这两种标志物通常存在于同一细胞中。在所有病例中，病毒抗原和RNA主要定位于感染细胞的细胞核。因此，在细胞和细胞内水平上，HDV RNA的复制与HDAg的表达密切相关，这类新的缺陷动物RNA病毒很可能在感染细胞的细胞核中复制。
Examination of a naturally infected human liver and experimentally infected chimpanzee and woodchuck livers byin situhybridization showed that hepatitis delta virus (HDV) RNA was restricted to hepatocytes. Genomic RNA was 20–30 times more abundant than antigenomic RNA and was predominantly single-stranded while antigenomic RNA was predominantly double-stranded. In acute delta hepatitis, viral RNA was a more reliable marker of virus infection in single cells than hepatitis delta antigen (HDAg) while in chronic hepatitis both markers were usually present in the same cell. In all cases, viral antigen and RNA were localized predominantly to the nuclei of infected cells. Thus, replication of HDV RNA is closely associated with HDAg expression at the cellular and intracellular level and it is likely that this new class of defective animal RNA viruses replicates in the nucleus of the infected cells.
影响因子：
3.7
作者：
Roger H. Miller;Patricia L. Marion;William S. Robinson
通讯作者：
Roger H. Miller;Patricia L. Marion;William S. Robinson