Allergic airway eosinophilia is suppressed in ovalbumin-sensitized brown Norway rats fed raffinose and α-linked galactooligosaccharide
Allergic airway eosinophilia is suppressed in ovalbumin-sensitized brown Norway rats fed raffinose and α-linked galactooligosaccharide
复制标题
DOI：
10.1093/jn/135.3.538
复制
发表时间：
2005-03-01
影响因子：
4.2
通讯作者：
Kawabata, J
中科院分区：
文献类型：
--
作者：
Sonoyama, K;Watanabe, H;Kawabata, J
我们最近发现，膳食棉子糖抑制过敏性气道嗜酸性粒细胞增多症的卵清蛋白致敏的布朗挪威大鼠。在本研究中使用该模型，我们比较了其他低聚糖与棉子糖的功效。将Brown Norway大鼠皮下免疫。在第0天用卵白蛋白，在第20天暴露于雾化卵白蛋白;在第21天获得支气管肺泡灌洗液。在Expt. 1，给大鼠喂食对照饮食或补充有不同低聚糖的饮食（50 g/kg饮食、棉子糖、α-连接的低聚半乳糖、低聚果糖和低聚木糖）。与喂食对照饲料的大鼠相比，喂食棉子糖和α-连接低聚半乳糖饲料的大鼠的液体中嗜酸性粒细胞数量显著降低。膳食低聚果糖和低聚木糖不影响气道嗜酸性粒细胞增多。在Expt. 2，腹膜内给予棉子糖和α-连接的低聚半乳糖，而不是低聚果糖和低聚木糖，抑制了喂食对照饮食的大鼠的气道嗜酸性粒细胞增多。在Expt. 3.在给予新霉素的盲肠切除大鼠中，饮食α-连接的低聚半乳糖抑制气道嗜酸性粒细胞增多。肺组织中白细胞介素（IL）-4和IL-5 mRNA水平的降低与气道嗜酸性粒细胞增多的抑制有关。我们建议，消化不良的寡糖不同的抑制作用，过敏性气道嗜酸性粒细胞增多症的卵蛋白致敏的布朗挪威大鼠，该效果似乎不介导的肠道菌群。
We recently found that dietary raffinose suppressed allergic airway eosinophilia in ovalbumin-sensitized Brown Norway rats. Using this model in the present study, we compared the efficacy of other oligosaccharides with that of raffinose. Brown Norway rats were immunized s.c. with ovalbumin on d 0 and exposed to aerosolized ovalbumin on d 20; broncho-alveolar lavage fluid was obtained on d 21. In Expt. 1, rats were fed a control diet or diets supplemented with different oligosaccharides (50 g/kg diet, raffinose, a-linked galactooligosaccharide, fructooligosaccharide, and xylooligosaccharide). The number of eosinophils in the fluid was significantly lower in rats fed raffinose and a-linked galactooligosaccharide diets than in those fed the control diet. Dietary fructooligosaccharide and xylooligosaccharide did not affect airway eosinophilia. In Expt. 2, i.p. administration of raffinose and a-linked galactooligosaccharide, but not fructooligosaccharide and xylooligosaccharide, suppressed airway eosinophilia in rats fed the control diet. In Expt. 3, suppression of airway eosinophilia by dietary a-linked galactooligosaccharide occurred in cecectomized rats administered neomycin. Reduced levels of interleukin (IL)-4 and IL-5 mRNA in lung tissue were associated with the suppression of airway eosinophilia. We propose that indigestible oligosaccharides differ in their suppressive effect on allergic airway eosinophilia in ovalbumin-sensitized Brown Norway rats and that the effect appears not to be mediated by intestinal microflora.