POLYNUCLEOTIDE PHOSPHORYLASE AND RIBONUCLEASE-II ARE REQUIRED FOR CELL VIABILITY AND MESSENGER-RNA TURNOVER IN ESCHERICHIA-COLI K-12
POLYNUCLEOTIDE PHOSPHORYLASE AND RIBONUCLEASE-II ARE REQUIRED FOR CELL VIABILITY AND MESSENGER-RNA TURNOVER IN ESCHERICHIA-COLI K-12
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DOI：
10.1073/pnas.83.1.120
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发表时间：
1986-01-01
影响因子：
11.1
通讯作者：
KUSHNER, SR
中科院分区：
文献类型：
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作者：
DONOVAN, WP;KUSHNER, SR
通过体外诱变分离出 RNase II (rnb) 的温度敏感等位基因，证明 RNase II 和多核苷酸磷酸化酶 (PNPase) 是大肠杆菌细胞活力和 RNA 周转所必需的。携带 pnp-7 和 rnb-500 等位基因（PNPase 缺陷和 RNase II 不耐热）的双突变菌株在转移到不允许的温度后 30 分钟内停止在 Luria 肉汤中生长。生长停止伴随着100-1500个核苷酸长的mRNA片段的积累。相比之下，在非允许温度下正常生长的单突变体和野生型对照菌株没有积累mRNA。在任何菌株中均未观察到 rRNA 模式的显着变化。
The isolation of a temperature-sensitive allele of RNase II (rnb) by in vitro mutagenesis has permitted the demonstration that RNase II and polynucleotide phosphorylase (PNPase) are required for cell viability and RNA turnover in Escherichia coli. Double-mutant strains carrying the pnp-7 and rnb-500 alleles (PNPase deficient and RNase II thermolabile) ceased growing in Luria broth within 30 min after shift to the nonpermissive temperature. Cessation of growth was accompanied by an accumulation of mRNA fragments 100-1500 nucleotides long. In contrast, a single-mutant and wild-type controls strains grew normally at the nonpermissive temperature did not accumulate mRNA. No significant changes in rRNA patterns were observed in any of the strains.