Role of PIN-mediated auxin efflux in apical hook development of Arabidopsis thaliana
Role of PIN-mediated auxin efflux in apical hook development of Arabidopsis thaliana
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DOI：
10.1242/dev.041277
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发表时间：
2010-02-15
期刊：
影响因子：
4.6
通讯作者：
Benkova, Eva
中科院分区：
文献类型：
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作者：
Zadnikova, Petra;Petrasek, Jan;Benkova, Eva
暗生长的拟南芥幼苗的顶钩是一种简单的结构，其在萌发后不久发育以在通过土壤出苗期间保护分生组织，并且在暴露于光时打开。顶钩的差异生长分三个连续的步骤进行，这三个步骤受多种激素（主要是生长素和乙烯）的调节。我们表明，顶钩通过这些发展阶段的进展取决于生长素的动态，不对称的分布，这是由PIN家族的生长素外排载体调节。几个PIN蛋白表现出特定的，部分重叠的空间和时间的表达模式，其亚细胞定位建议生长素通量在钩的发展。遗传操作的个人PIN活动干扰不同阶段的钩发展，这意味着特定的PIN基因组合所需的顶钩通过发展阶段的进展。此外，乙烯可能通过延长形成期和强烈抑制维持期来调节顶钩的发育。这种乙烯效应部分是通过调节PIN依赖的生长素流出和生长素信号传导介导的。
The apical hook of dark-grown Arabidopsis seedlings is a simple structure that develops soon after germination to protect the meristem tissues during emergence through the soil and that opens upon exposure to light. Differential growth at the apical hook proceeds in three sequential steps that are regulated by multiple hormones, principally auxin and ethylene. We show that the progress of the apical hook through these developmental phases depends on the dynamic, asymmetric distribution of auxin, which is regulated by auxin efflux carriers of the PIN family. Several PIN proteins exhibited specific, partially overlapping spatial and temporal expression patterns, and their subcellular localization suggested auxin fluxes during hook development. Genetic manipulation of individual PIN activities interfered with different stages of hook development, implying that specific combinations of PIN genes are required for progress of the apical hook through the developmental phases. Furthermore, ethylene might modulate apical hook development by prolonging the formation phase and strongly suppressing the maintenance phase. This ethylene effect is in part mediated by regulation of PIN-dependent auxin efflux and auxin signaling.