In vitro formation and characterization of a perfusable three-dimensional tubular capillary network in microfluidic devices
In vitro formation and characterization of a perfusable three-dimensional tubular capillary network in microfluidic devices
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DOI：
10.1039/c2lc40131b
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Jeon, Noo Li
中科院分区：
文献类型：
--
作者：
Yeon, Ju Hun;Ryu, Hyun Ryul;Jeon, Noo Li
This paper describes the in vitro formation and characterization of perfusable capillary networks made of human umbilical vein endothelial cells (HUVECs) in microfluidic devices (MFDs). Using this platform, an array of three-dimensional (3D) tubular capillaries of various dimensions (50-150 mu m in diameter and 100-1600 mu m in length) can be formed reproducibly. To generate connected blood vessels, MFDs were completely filled with fibrin gel and subsequently processed to selectively leave behind gel structures inside the bridge channels. Following gel solidification, HUVECs were coated along the gel walls, on opposite ends of the patterned 3D fibrin gel. After 3-4 days, HUVECs migrating into the fibrin gel from opposite ends fused with each other, spontaneously forming a connected vessel that expressed tight junction proteins (e. g., ZO-1), which are characteristic of post-capillary venules. With ready access to a perfusable capillary network, we demonstrated perfusion of the vessels and imaged red blood cells (RBCs) and beads flowing through them. The results were reproducible (similar to 50% successful perfusable capillaries), consistent, and could be performed in a parallel manner (9 devices per well plate). Additionally, compatibility with high resolution live-cell microscopy and the possibility of incorporating other cell types makes this a unique experimental platform for investigating basic and applied aspects of angiogenesis, anastomosis, and vascular biology.
