Cortical bitufted, horizontal, and Martinotti cells preferentially express and secrete reelin into perineuronal nets, nonsynaptically modulating gene expression
Cortical bitufted, horizontal, and Martinotti cells preferentially express and secrete reelin into perineuronal nets, nonsynaptically modulating gene expression
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DOI：
10.1073/pnas.96.6.3217
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发表时间：
1999-03-16
影响因子：
11.1
通讯作者：
Caruncho, HJ
中科院分区：
文献类型：
--
作者：
Pesold, C;Liu, WS;Caruncho, HJ
Reelin (Reln) is a protein with some structural analogies with other extracellular matrix proteins that functions in the regulation of neuronal migration during the development of cortical laminated structures. In the cortex of adult animals, Rein is expressed primarily in gamma-aminobutyric acid (GABA)ergic neurons and is secreted into perineuronal nets. However, only 50-60% of GABAergic interneurons express Rein. We have characterized this subpopulation of cortical GABAergic neurons that expresses Rein by using two strategies: (i) a double immunolabeling procedure to determine the colocalization of Rein with neuropeptides and Ca2+-binding proteins and (ii) a combination of Golgi staining and Rein immunolabeIing to determine the morphology of the rat cortical cells that store Rein, Many interneurons that express Neuropeptide Y (NPY) or somatostatin (but none of those that express parvalbumin) are Reln-immunopositive, A small population of calbindin-positive interneurons and very few calretinin-positive cells express Rein immunopositivity. Golgi staining revealed that layer I horizontal cells, layer Il-V bitufted neurons, and some deep cortical layer Martinotti cells express Rein. Basket and chandelier cells are often immunopositive to parvalbumin, but never to Rein, Although Rein is secreted by GABAergic neurons, its target are not the GABA receptors, but rather may be extrasynaptically located in perineuronal nets and concerned with the modulation of neuronal plasticity, Dab1, the target adapter protein that presumably mediates transcription regulation via the extrasynaptic actions of Rein, is expressed predominantly in pyramidal neurons, but it can also be detected in a small population of GABAergic neurons that are neither horizontal nor bitufted neurons.
