Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana.
Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana.
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拟南芥乙烯受体 ETR1 催化结构域的克隆、过表达、纯化和初步 X 射线分析。
DOI：
10.1107/s174430911302842x
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
J. Mueller
中科院分区：
文献类型：
--
作者：
S. Panneerselvam;H. Kaljunen;J. Mueller
乙烯是一种气体植物激素，它控制植物生长和发育的许多方面。它被膜结合受体感知，与细菌双组分系统相似。拟南芥ETR1组氨酸激酶结构域的催化和ATP结合结构域已被克隆、过表达和结晶。蛋白质与各种核苷酸一起结晶。在ADP存在下获得的晶体属于空间群I222或I2（1）2（1）2（1），每个不对称单元具有一个分子。他们衍射X射线辐射到超过1.85毫米的分辨率。
Ethylene is a gaseous plant hormone which controls many aspects of plant growth and development. It is perceived by membrane-bound receptors with a similarity to bacterial two-component systems. The catalytic and ATP-binding domain of the histidine kinase domain of ETR1 from Arabidopsis thaliana has been cloned, overexpressed and crystallized. The protein was crystallized together with various nucleotides. Crystals obtained in the presence of ADP belonged to space group I222 or I2(1)2(1)2(1) with one molecule per asymmetric unit. They diffracted X-ray radiation to beyond 1.85 Å resolution.
影响因子：
1.6
作者：
Studier, FW
通讯作者：
Studier, FW