Chemical synthesis of capped oligoribonucleotides, m7G5'pppAUG and m7G5'pppAUGACC
Chemical synthesis of capped oligoribonucleotides, m7G5'pppAUG and m7G5'pppAUGACC
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加帽寡核糖核苷酸 m7G5pppAUG 和 m7G5pppAUGACC 的化学合成
DOI：
10.1246/bcsj.58.850
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发表时间：
1985
影响因子：
4
通讯作者：
T. Hata
中科院分区：
文献类型：
--
作者：
M. Sekine;S. Nishiyama;T. Kamimura;Y. Osaki;T. Hata
全保护的paaug（15）和paaugacc（21）采用磷酸三酯法合成，其中苯基硫基作为内部和5 '端磷酸保护基团。（15）和（21）经碱性处理得到部分解封低聚物（32）和（33），在咪唑存在下，用硝酸银活化，用盖层试剂（1a）缩合，得到被酸不稳定保护基团保护的三聚体和六聚体，即4,4,' 4″-三甲氧基三甲基（TMTr）， 4-单甲氧基三甲基（MMTr），四氢吡喃-2-基（THP）和甲氧基亚甲基（mM）基团。用稀HCl溶液解封合得到m7G5 ' pppaaug和m7G5 ' pppaaugacc，用高效液相色谱法纯化，并用酶学分析对其进行表征。
The fully-protected pAUG (15) and pAUGACC (21) were synthesized by the phosphotriester approach where the phenylthio group was employed as the internal and 5′-terminal phosphate-protecting groups. The partially unblocked oligomers (32) and (33), obtained by alkaline treatment of (15) and (21), were condensed with a capping reagent (1a) in the presence of imidazole by activation with silver nitrate to afford the capped trimer and hexamer protected with the acid-labile protecting groups, i.e., 4,4,′4″-trimethoxytrityl (TMTr), 4-monomethoxytrityl (MMTr), tetrahydropyran-2-yl (THP), and methoxymethylene (mM) groups. The protected capped oligomers were unblocked by a dilute HCl solution to afford m7G5′ pppAUG and m7G5′ pppAUGACC, which were purified by HPLC and characterized by enzyme assays.