Production of L-lactic acid from metabolically engineered strain of Enterobacter aerogenes ATCC 29007
Production of L-lactic acid from metabolically engineered strain of Enterobacter aerogenes ATCC 29007
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DOI：
10.1016/j.enzmictec.2017.03.003
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发表时间：
2017-07-01
影响因子：
3.4
通讯作者：
Kim, Seung Wook
中科院分区：
文献类型：
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作者：
Thapa, Laxmi Prasad;Lee, Sang Jun;Kim, Seung Wook
在本研究中，研究了产气杆菌 ATCC 29007 代谢工程菌株的 L-乳酸生产情况。工程菌株产气杆菌 SUMI01 (Delta pta) 是通过从产气杆菌 ATCC 29007 染色体上删除磷酸乙酰转移酶 (pta) 基因而产生的，并通过菌落 PCR 证实了删除。在优化的发酵条件下，37℃、pH 6下发酵84 h，工程菌产气杆菌SUMI01（Delta pta）以100 g/L甘露醇为碳源进行烧瓶发酵，产L-乳酸为40.05 g/L，而野生型为20.70 g/L。生物反应器分批发酵结束时，L-乳酸产量达到46.02 g/L，利用112.32 g/L甘露醇，产量为0.41 g/g。这是第一份关于从肠杆菌属中生产 L-乳酸的报告。我们相信，这一结果可能为进一步改造肠杆菌菌株以提高 L-乳酸产量提供有价值的指导。 (C) 2017 Elsevier Inc. 保留所有权利。
In this study, L-lactic acid production was investigated from metabolically engineered strain of E. aerogenes ATCC 29007. The engineered strain E. aerogenes SUMI01 (Delta pta) was generated by the deletion of phosphate acetyltransferase (pta) gene from the chromosome of E. aerogenes ATCC 29007 and deletion was confirmed by colony PCR. Under the optimized fermentation conditions, at 37 degrees C and pH 6 for 84 h, the L-lactic acid produced by engineered strain E. aerogenes SUMI01 (Delta pta) in flask fermentation using 100 g/L mannitol as the carbon source was 40.05 g/L as compared to that of the wild type counterpart 20.70 g/L. At the end of the batch fermentation in bioreactor the production of L-lactic acid reached to 46.02 g/L and yield was 0.41 g/g by utilizing 112.32 g/L mannitol. This is the first report regarding the production of L-lactic acid from Enterobacter species. We believe that this result may provide valuable guidelines for further engineering Enterobacter strain for the improvement of L-lactic acid production. (C) 2017 Elsevier Inc. All rights reserved.