The cyclin-dependent kinase 11p46 isoform interacts with RanBPM
The cyclin-dependent kinase 11p46 isoform interacts with RanBPM
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DOI：
10.1016/j.bbrc.2003.08.116
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发表时间：
2003-10-10
影响因子：
3.1
通讯作者：
Nelson, M
中科院分区：
文献类型：
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作者：
Mikolajczyk, M;Shi, JQ;Nelson, M
我们利用酵母双杂交系统鉴定了Ran结合蛋白(RanBPM)是caspase加工的细胞周期蛋白依赖蛋白11(CDK11(P46))的C末端结构域的相互作用伙伴。CDK11(P110)蛋白激酶是细胞周期蛋白依赖性蛋白激酶超家族的成员。在星形孢菌素、Fas-和肿瘤坏死因子a诱导的细胞凋亡过程中，较大的CDK11(P110)亚型产生caspase处理的活化的CDK11(P46)。CDK11(P46)在人细胞中异位表达时可促进细胞凋亡。然而，CDK11(P46)的信号转导机制尚不清楚。在这项研究中，我们证明了CDK11(P46)在体外和人类细胞中直接与RanBPM相互作用。RanBPM含有一个保守的SPRY结构域(在sp1A和RyR中重复)，定位于细胞核和细胞质。RanBPM的SPRY结构域负责CDK11(P46)与RanBPM之间的联系。此外，我们还发现CDK11(46)可以磷酸化RanBPM。(C)2003 Elsevier Inc.保留所有权利。
We identified Ran-binding protein (RanBPM) as an interacting partner of the caspase-processed C-terminal domain of cyclin-dependent kinase 11 (CDK11(p46)) by using the yeast two-hybrid system. CDK11(p110) protein kinases are members of the cyclin-dependent kinase superfamily. During staurosporine-, Fas-, and tumor necrosis factor a-induced apoptosis caspase-processed activated CDK11(p46) is generated from larger CDK11(p110) isoforms. CDK11(p46) promotes apoptosis when it is ectopically expressed in human cells. However, the mechanism of signal transduction through CDK11(p46) is still unclear. In this study, we demonstrate that CDK11(p46) directly interacts with RanBPM in vitro and in human cells. RanBPM contains a conserved SPRY (repeats in sp1A and Ryr) domain and is localized both in the nucleus and cytoplasm. The SPRY domain of RanBPM is responsible for the association between CDK11(p46) and RanBPM. Furthermore, we show that CDK11(46) phosphorylates RanBPM. (C) 2003 Elsevier Inc. All rights reserved.