hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.
hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.
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DOI：
10.1016/j.ygeno.2006.11.012
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发表时间：
2007-03
期刊：
影响因子：
4.4
通讯作者：
Vidal M
中科院分区：
文献类型：
--
作者：
Lamesch P;Li N;Milstein S;Fan C;Hao T;Szabo G;Hu Z;Venkatesan K;Bethel G;Martin P;Rogers J;Lawlor S;McLaren S;Dricot A;Borick H;Cusick ME;Vandenhaute J;Dunham I;Hill DE;Vidal M
完整的克隆蛋白质编码开放式阅读框（ORF）或ORFEOMES是大规模蛋白质组学和系统生物学研究的重要工具。在这里，我们描述了Human Orfeome版本3.1（Horfeome v3.1），目前是全长人类ORF的最大公开资源（可在www.openbiosystems.com上获得）。该系列由Gateway重组克隆产生，包含12,212个ORF，代表10,214个人类基因，对应于原始Horfeome V1.1的51％膨胀。建造了一个在线人类Orfeome数据库HORFDB，并用作所有克隆人类ORF（http://horfdb.dfci.harvard.edu）的中央存储库。原始Orfeome资源的这种扩展大大增加了大规模蛋白质组学研究的潜在实验搜索空间，这将导致生成更全面的数据集。
Complete sets of cloned protein-encoding open reading frames (ORFs), or ORFeomes, are essential tools for large-scale proteomics and systems biology studies. Here we describe human ORFeome version 3.1 (hORFeome v3.1), currently the largest publicly available resource of full-length human ORFs (available at www.openbiosystems.com). Generated by Gateway recombinational cloning, this collection contains 12,212 ORFs, representing 10,214 human genes, and corresponds to a 51% expansion of the original hORFeome v1.1. An online human ORFeome database, hORFDB, was built and serves as the central repository for all cloned human ORFs (http://horfdb.dfci.harvard.edu). This expansion of the original ORFeome resource greatly increases the potential experimental search space for large-scale proteomics studies, which will lead to the generation of more comprehensive datasets.