Inhibition of lysozyme lytic activity by Ivy derived from Photobacterium damselae subsp. piscicida
Inhibition of lysozyme lytic activity by Ivy derived from Photobacterium damselae subsp. piscicida
复制标题
源自发光杆菌亚种的常春藤对溶菌酶裂解活性的抑制。
DOI：
10.1016/j.fsi.2022.04.012
复制
发表时间：
2022
影响因子：
4.7
通讯作者：
Hikima Jun-ichi
中科院分区：
文献类型：
--
作者：
Nishihara Aki;Morimoto Natsuki;Sumiyoshi Takechiyo;Yasumoto Shinya;Kondo Masakazu;Kono Tomoya;Sakai Masahiro;Hikima Jun-ichi
一种假结核病病原体，发光杆菌杀鱼亚种（Pdp），已造成巨大的经济损失，在日本的黄尾水产养殖。Ivy基因已在Pdp质粒中发现，并且已经提出它可能帮助细菌在与动物宿主相互作用期间逃避溶菌酶介导的裂解。然而，Pdp衍生的常春藤（Ivy-Pdp）的溶菌酶抑制活性尚不清楚，也不清楚它是否作为宿主生物防御的毒力因子。本研究通过表达和纯化重组Ivy-Pdp蛋白（rIvy-Pdp），研究Ivy-Pdp对溶菌酶的抑制作用。rIvy-Pdp蛋白对鸡蛋白色溶菌酶的抑制作用呈浓度依赖性，且在不同温度和pH条件下的抑制效果相似。黄尾鱼（Pdp的宿主物种）、日本比目鱼和尼罗罗非鱼的血清和皮肤粘液显示出溶菌活性。相反，rIvy-Pdp的加入抑制了这些鱼类血清中的裂解活性。特别是，它显着抑制尼罗罗非鱼的血清和皮肤粘液中的溶解活性。在这些结果的基础上，我们认为Ivy-Pdp是一种温度和pH稳定的溶菌酶抑制剂。此外，Ivy-Pdp抑制溶菌酶的裂解活性，这是参与宿主的生物防御。综上所述，我们推断Ivy-Pdp是Pdp感染宿主时降低C型溶菌酶杀菌能力的一个重要因素。
A pseudotuberculosis pathogen, Photobacterium damselae subsp.piscicida(Pdp), has caused enormous economic damage to yellowtail aquaculture in Japan. The Ivy gene has been discovered in plasmid ofPdp, and it has been proposed that it may help bacteria evade lysozyme-mediated lysis during interaction with an animal host. However, the lysozyme-inhibiting activity ofPdp-derived Ivy (Ivy-Pdp) is unknown, and it is unclear whether it acts as a virulence factor for host biophylaxis. In this study, the inhibitory effect of Ivy-Pdp on lysozyme was evaluated by expressing and purifying the recombinant Ivy-Pdp protein (rIvy-Pdp). The rIvy-Pdp protein inhibited hen egg white lysozyme activity in an rIvy-Pdp-concentration-dependent manner, and its inhibitory effect was similar under different temperature and pH conditions. The serum and skin mucus of the yellowtail (which is the host species of Pdp), Japanese flounder, and Nile tilapia showed bacteriolytic activity. In contrast, the addition of rIvy-Pdp inhibited the lytic activity in the serum of these fish species. In particular, it significantly inhibited lytic activity in the serum and skin mucus of Nile tilapia. On the basis of these results, we suggest that Ivy-Pdp is a temperature- and pH-stable lysozyme inhibitor. Additionally, Ivy-Pdp inhibited the lytic activity of lysozyme, which is involved in host biophylaxis. In summary, we inferred that Ivy-Pdp is an important factor that diminishes the sterilization ability of C-type lysozyme whenPdpinfects the host.