Highly efficient near-infrared organic dots based on novel AEE fluorogen for specific cancer cell imaging
Highly efficient near-infrared organic dots based on novel AEE fluorogen for specific cancer cell imaging
复制标题
基于新型 AEE 荧光剂的高效近红外有机点，用于特定癌细胞成像
DOI：
10.1039/c5ra04669f
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发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Tian Wenjing
中科院分区：
文献类型：
--
作者：
Zhang Yan;Chang Kaiwen;Xu Bin;Chen Jinlong;Yan Lulin;Ma Suqian;Wu Changfeng;Tian Wenjing
以两亲性聚合物聚(苯乙烯-马来酸酐)(PSMA)为共包覆基质，以新型近红外发射小分子氟化物(DPPBPA)为核，制备了具有高荧光量子效率的近红外发光有机点(AEE点)。结果表明，所制备的PSMA纳米粒子尺寸小，斯托克斯位移304 nm，荧光量子效率高达20%。链霉亲和素点是通过链霉亲和素与PSMA点表面的羧基偶联而得到的。这些链霉亲和素能够有效和特异地标记靶细胞，而不需要任何非特异性结合，如MCF-7细胞。除了可以忽略的细胞毒性，近红外发射的AEE点有望成为未来生物成像应用的红色荧光探针。
Near-infrared emissive organic dots with a high fluorescence quantum efficiency (AEE dots) are prepared by using an amphiphilic polymer poly(styrene-co-maleic anhydride) (PSMA) as the co-encapsulation matrix and a novel small molecule fluorogen (DPPBPA) with high near-infrared emission as the core. The PSMA dots show small particle size of about 20 nm, a large Stokes shift of 304 nm and really high fluorescence quantum efficiency of 20%. The streptavidin-dots are obtained by conjugating streptavidin to carboxyl groups on the surface of PSMA dots. These streptavidin-dots can effectively and specifically label the target cell without any nonspecific binding, such as MCF-7 cells. Together with the negligible cytotoxicity, the near-infrared emissive AEE dots are promising red fluorescent probes for future bioimaging applications.