Phenotypic characteristics of a slow-growing, nongerminating variant of Candida albicans.
Phenotypic characteristics of a slow-growing, nongerminating variant of Candida albicans.
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白色念珠菌生长缓慢、不发芽变种的表型特征。
DOI：
10.1099/00221287-132-8-2359
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Dabrowa,N
中科院分区：
文献类型：
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作者：
Howard,DH;Zeuthen,ML;Dabrowa,N
描述了常见研究的白色念珠菌菌株的生长缓慢、不发芽变体的一些表型特征。该变体是偶然分离出来的。发生率约为0·1%，回复率约为每106个细胞1个。菌落大小通常小于亲本，并且酵母细胞往往不会彼此分离，从而形成链状细胞链（假菌丝）。在标准条件下，液体振荡培养中小菌落变体的产生时间约为亲本菌株的两倍。抗霉素 A 抑制了该变体的生长，表明小集落形式并不是细胞色素系统缺陷的结果。该变体的集落大小不受氯苯并三唑的影响，这表明腺嘌呤代谢不参与小集落表型。高浓度的可利用碳水化合物并不能缓解假菌丝生长模式，这意味着酵母细胞的链状微观外观不仅仅是C.分离株正常生长模式的夸大。白色念珠菌但更可能代表细胞分离步骤中有缺陷的细胞周期突变体的生长。变体和亲本的细胞质蛋白非常相似，尽管每个变体都显示出一些独特的肽。
Some of the phenotypic characteristics of a slow-growing, nongerminating variant of a commonly studied strain ofCandida albicansare described. The variant arose as a chance isolate. The rate of occurrence was about 0·1% and the reversion rate was about 1 per 106cells. The colony size was typically smaller than that of the parent and the yeast cells tended not to separate from one another so that catenulate strands of cells (pseudohyphae) were formed. Under standard conditions the generation time of the small-colony variant in liquid shake cultures was about twice that of the parental strain. Growth of the variant was suppressed by antimycin A, indicating that the small colony form was not the consequence of a defect in the cytochrome system. The colony size of the variant was not influenced by chlorobenzotriazole, which suggested that adenine metabolism was not involved in the small-colony phenotype. The pseudohyphal growth pattern was not relieved by high concentrations of utilizable carbohydrates, which means the catenulate microscopic appearance of the yeast cells was not simply an exaggeration of the normal growth pattern of isolates ofC. albicansbut more probably represented the growth of a cell-cycle mutant defective at the cell separation step. The cytoplasmic proteins of the variant and the parent were very similar though some unique peptides were displayed by each.