Myoblast-acellular skeletal muscle matrix constructs guarantee a long-term repair of experimental full-thickness abdominal wall defects
Myoblast-acellular skeletal muscle matrix constructs guarantee a long-term repair of experimental full-thickness abdominal wall defects
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DOI：
10.1089/ten.2006.12.1929
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发表时间：
2006-07-01
影响因子：
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通讯作者：
Paolo Parnigotto, Pier
中科院分区：
文献类型：
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作者：
De Coppi, Paolo;Bellini, Silvia;Paolo Parnigotto, Pier
为了获得一个有价值的治疗先天性肌肉缺损，细胞-基质结构组成的卫星细胞衍生的成肌细胞（XY核型）接种在肌肉脱细胞基质，用于修复先前创建的全层缺损的腹壁的18个1个月大的雌性刘易斯大鼠。通过去污剂-酶法获得的无细胞腹部基质对碱性成纤维细胞生长因子和转化生长因子-β均呈阳性，并且能够支持体外细胞粘附。所有动物均在手术中存活，没有感染或植入物排斥的迹象，并在手术后1、3或9个月人道处死。植入物保存良好，与宿主组织整合，并保持其原始尺寸和厚度，直至9个月。囊泡乙酰胆碱转运体在术后1个月开始在肌纤维表面表达。最后，植入的男性成肌细胞内的补丁，直到9个月，所证明的SrY mRNA的表达和Y染色体探针信号的存在。这些结果使我们能够得出结论，细胞-基质构建体可能是修复肌肉缺损的一种有前途的方法，因为它们在体内由骨骼肌细胞和神经元件重新填充，并在长期内保持其结构完整性。
To obtain a valuable treatment of congenital muscle defect, cell-matrix constructs composed of satellite cell-derived myoblasts (XY karyotype) seeded on muscle acellular matrices were used to repair a previously created full-thickness defect of abdominal wall of 18 1-month-old female Lewis rats. Acellular abdominal matrices, obtained by a detergent-enzymatic method, were positive for both basic fibroblast growth factor and transforming growth factor-beta, and were able to support in vitro cell adhesion. All animals survived the surgery, without signs of infection or implant rejection, and were humanely killed at 1, 3, or 9 months after surgery. The implants appeared well preserved, were integrated in the host tissue, and maintained their original dimension and thickness until 9 months. Vesicular acetylcholine transporter was expressed on the surface of muscle fibers from 1 month postsurgery. Finally, implanted male myoblasts were present inside the patches until 9 months, as demonstrated by the expression of SrY mRNA and by the presence of Y chromosome probe signal. These results allow us to conclude that cell-matrix constructs could represent a promising approach to the repair of muscle defects, because they are repopulated in vivo by skeletal muscle cells and nervous elements and maintain their structural integrity over the long term.