A Method of Staining Nucleoli of Cells in Fresh Benign and Malignant Tissues
A Method of Staining Nucleoli of Cells in Fresh Benign and Malignant Tissues
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新鲜良性和恶性组织中细胞核仁的染色方法
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发表时间：
1948
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通讯作者：
W. Ayres
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作者：
W. Ayres
随着对各种渗出液和体液中恶性细胞诊断的兴趣日益增加，一种能详细显示某些核仁变化的方法可能具有价值。本文旨在介绍这种方法，并简要讨论良、恶性组织中核仁的变化，同时指出这种方法在癌症诊断中的潜在应用价值。天青C法是基于观察到新鲜组织细胞的核仁在暴露于某些染料时选择性染色。Naegeli（17，18）指出，淋巴细胞和成髓细胞的核仁通常用Pappenheim、Nakanishi、Sabrazes、Schilling-Torgau和Cesper-Demel描述的超活体技术染色良好。这些方法使用亚甲蓝、亮甲酚蓝或吉姆萨染色。特别是Quensel（21，22），使用亚甲蓝-镉和苏丹III-镉的染色混合物研究了胸腔和腹水中恶性细胞的核仁。他指出，用他的方法染色核仁比用任何其他方法都好。MacCarty（13，14）和MacCarty和Haumeder（15）在对良性和恶性细胞的核仁和核-核仁比的广泛研究中，推荐Terry或Unnas的多色亚甲蓝用于冷冻切片中这些小体的染色。MacCarty指出，核仁在新鲜组织中比在固定组织中显示得更好。Guzman（7，8）在外周血淋巴细胞和单核细胞核仁的研究中，开发了一种利用稀释的Leishman或Giemsa染色的方法。Von Haam和亚历山大（25，26）在他们对恶性肿瘤细胞学的研究中，开发了一种用高稀释度（1：10，000）的甲苯胺蓝对悬浮的新鲜未固定细胞的核仁进行染色的方法。在上述大多数方法中，甲基-
With the increasing interest in the diagnosis of malignant cells in various exudates and body fluids, a method demonstrating certain nucleolar changes in considerable detail may be of value. I t is t he purpose of this paper to present such a method and to discuss briefly the nucleolar changes in benign and malignant tissues and also to point out the potential applicability of this method as an aid in the diagnosis of cancer. The azure C method is based on the observation that nucleoli of cells of fresh tissues when exposed to certain dyes, stain electively. Naegeli (17, I8) states that the nucleoli of lymphocytes and myeloblasts often stain well with supravital technics as described by Pappenheim, Nakanishi, Sabrazes, Schilling-Torgau and Cesaris-Demel. These methods utilize methylene blue, brilliant cresyl blue or Giemsa stain. Quensel (21, 22), in particular, has studied the nucleoli of malignant cells in pleural and ascitic fluids using a staining mixture of methylene-blue-cadmium and Sudan III-cadmium. He states that with his method the nucleoli stain better than with any other method. MacCarty (13, 14), and MacCarty and Haumeder (15), in extensive work on the nucleoli and the nuclear-nucleolar ratio of benign and malignant cells, recommend Terry's or Unnas' polychrome methylene blue for the staining of these bodies in frozen sections. MacCarty states that the nucleolus may be better demonstrated in fresh tissues than in fixed tissues. Guzman (7, 8), in a study of the nucleoli of lymphocytes and monocytes of the peripheral blood, developed a method utilizing diluted Leishman or Giemsa stain. Von Haam and Alexander (25, 26), in their work on the cytology of malignant tumors, developed a method of staining nucleoli of fresh unfixed cells in suspension, with high dilutions (1 : 10,000) of toluidine blue. In most of the methods mentioned above, methy-