Phloem Protein Partners of Cucurbit aphid borne yellows virus: Possible Involvement of Phloem Proteins in Virus Transmission by Aphids
Phloem Protein Partners of Cucurbit aphid borne yellows virus: Possible Involvement of Phloem Proteins in Virus Transmission by Aphids
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DOI：
10.1094/mpmi-23-6-0799
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发表时间：
2010-06-01
影响因子：
3.5
通讯作者：
Brault, V.
中科院分区：
文献类型：
--
作者：
Bencharki, B.;Boissinot, S.;Brault, V.
波罗病毒是严格由食韧皮部的蚜虫以循环和非繁殖模式传播的植物病毒。在摄入过程中，蚜虫会在筛管中采集病毒粒子和汁液。因此，任何与病毒体结合的汁液蛋白都会被昆虫获取，并可能参与传播过程。通过对从黄瓜中收集的汁液蛋白进行体外病毒覆盖测定，我们观察到大约 20 种蛋白能够与葫芦蚜传黄病毒 (CABYV) 的纯化颗粒结合。其中，通过质谱鉴定了八种蛋白质。通过使用来自拟南芥的纯化直系同源 PP2 蛋白，进一步研究了属于 PP2 样家族（在葫芦汁中发现的主要凝集素）的两种候选物在蚜虫传播中的作用。将这些蛋白质添加到蚜虫人工饲料的病毒悬浮液中大大增加了病毒传播率。这种转变与昆虫细胞中病毒基因组数量的增加以及体外病毒粒子稳定性的增加相关。令人惊讶的是，在蚜虫饮食中添加无关蛋白质后，还监测到病毒传播率的增加，这表明饮食中任何浓度足够高且与病毒颗粒一起获得的可溶性蛋白质都可以刺激病毒传播。
Poleroviruses are phytoviruses strictly transmitted by phloem-feeding aphids in a circulative and nonpropagative mode. During ingestion, aphids sample virions in sieve tubes along with sap. Therefore, any sap protein bound to virions will be acquired by the insects and could potentially be involved in the transmission process. By developing in vitro virus-overlay assays on sap proteins collected from cucumber, we observed that approximately 20 proteins were able to bind to purified particles of Cucurbit aphid borne yellows virus (CABYV). Among them, eight proteins were identified by mass spectrometry. The role of two candidates belonging to the PP2-like family (predominant lectins found in cucurbit sap) in aphid transmission was further pursued by using purified orthologous PP2 proteins from Arabidopsis. Addition of these proteins to the virus suspension in the aphid artificial diet greatly increased virus transmission rate. This shift was correlated with an increase in the number of viral genomes in insect cells and with an increase of virion stability in vitro. Surprisingly, increase of the virus transmission rate was also monitored after addition of unrelated proteins in the aphid diet, suggesting that any soluble protein at sufficiently high concentration in the diet and acquired together with virions could stimulate virus transmission.