Polyphosphate Derived from Lactobacillus brevis Inhibits Colon Cancer Progression Through Induction of Cell Apoptosis.
Polyphosphate Derived from Lactobacillus brevis Inhibits Colon Cancer Progression Through Induction of Cell Apoptosis.
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发表时间：
2016-02
影响因子：
2
通讯作者：
Aki Sakatani;M. Fujiya;Nobuhiro Ueno;Shin Kashima;J. Sasajima;K. Moriichi;K. Ikuta;H. Tanabe;Y. Kohgo
中科院分区：
文献类型：
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作者：
Aki Sakatani;M. Fujiya;Nobuhiro Ueno;Shin Kashima;J. Sasajima;K. Moriichi;K. Ikuta;H. Tanabe;Y. Kohgo
虽然益生菌已知具有抗肿瘤活性，但很少有细菌来源的抗肿瘤分子被鉴定出来。本研究探索了从短乳杆菌SBL8803(L.brevis 8803)中提取的一种抗肿瘤分子及其作用机制。细胞存活率和细胞凋亡率分别用硫代罗丹明B比色法和末端脱氧核苷酸转移酶dUTP法检测。Western blotting检测磷酸化细胞外信号调节激酶(ERK)和裂解多聚ADP核糖聚合酶(PARP)的表达。短链杆菌8803条件培养液对SW620细胞活力有抑制作用，其作用可因条件培养液中多聚磷的降解而减弱。异种移植模型显示，聚P抑制SW620细胞的生长。Poly P通过激活ERK通路诱导SW620细胞凋亡。相反，在来自正常小鼠的原代培养细胞中，Poly P不影响细胞活力。益生菌衍生的聚P有望成为一种比传统药物副作用更少的抗肿瘤药物。
Although probiotics are known to have antitumor activity, few bacteria-derived antitumor molecules have been identified. The present study explored an antitumor molecule derived from Lactobacillus brevis SBL8803 (L. brevis 8803) and the mechanisms that underlie its effects. Cell viability and apoptosis were assessed by a sulforhodamine B assay and terminal deoxynucleotidyl transferase dUTP staining, respectively. Phosphorylated extracellular signal-regulated kinase (ERK) and cleaved poly ADP-ribose polymerase (PARP) expression were detected by western blotting. The conditioned medium of L. brevis 8803 inhibited SW620 cells viability and the effect was reduced by the degradation of polyphosphate (poly P) in the conditioned medium. A xenograft model showed that poly P inhibited the growth of SW620 cells. Poly P induced the apoptosis of SW620 cells through activation of the ERK pathway. In contrast, in primary cultured cells derived from normal mouse, poly P did not affect cell viability. Probiotic-derived poly P is expected to be an antitumor drug with fewer adverse effects than conventional drugs.